Integrated molecular diagnosis apparatus
Inventors
Lee, Kook Nyung • Lee, Hyun Young • Cho, Sang Gyu • Hong, Dong Ki • Cheon, Jeong Hun • Kang, Joo Sung • KANG, Hye Lim • Yun, Seo Jin
Assignees
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Publication Number
US-12427515-B2
Publication Date
2025-09-30
Expiration Date
Abstract
An integrated molecular diagnosis apparatus including a buffer tube into which a sample collection tool collecting a sample is inserted, the buffer preparing a sample solution that contains nucleic acid extracted from the collected sample, a cartridge combined with the buffer tube and supplied with the sample solution, the cartridge transporting the sample solution to a reaction chamber through a fluid channel and performing a nucleic acid amplification reaction, and a diagnosis module main body detachably combined with the cartridge, the diagnosis module main body supplying heat at a predetermined temperature to the reaction chamber, detecting the nucleic acid amplification reaction, and determining whether or not a diagnosis target is present.
Core Innovation
The integrated molecular diagnosis apparatus includes a buffer tube inserted with a sample collection tool collecting a sample, and the buffer tube prepares a sample solution containing nucleic acid extracted from the collected sample. The prepared sample solution is supplied to a cartridge combined with the buffer tube, and the cartridge transports the sample solution to a reaction chamber through a fluid channel and performs a nucleic acid amplification reaction.
A diagnosis module main body detachably combines with the cartridge. The diagnosis module main body supplies heat at a predetermined temperature to the reaction chamber, detects nucleic acid amplification, and determines whether or not a diagnosis target is present.
The cartridge includes a plate-shaped cartridge body having a front surface and a rear surface, with a tube accommodation body formed on the front surface to insert the buffer tube, an inlet port formed through the rear surface, and a fluid channel transporting the sample solution toward an outlet port. The cartridge further includes a reaction chamber formed within the fluid channel on the rear surface and containing a pre-injected reagent, and an outlet-port plugging member inserted into the outlet port that allows air to pass through and blocks passage of the sample solution.
Claims Coverage
The partial content provides one independent claim and dependent refinements. The independent claim combines buffer tube sample preparation, cartridge transport and reaction chamber architecture, and a detachable diagnosis module for heating, amplification detection, and determination of a diagnosis target.
Buffer tube prepares nucleic-acid sample solution from collected sample
A buffer tube into which a sample collection tool collecting a sample is inserted, the buffer tube preparing a sample solution that contains nucleic acid extracted from the collected sample.
Cartridge transports sample solution via fluid channel to reaction chamber for nucleic-acid amplification
A cartridge combined with the buffer tube and supplied with the sample solution, the cartridge transporting the sample solution to a reaction chamber through a fluid channel and performing a nucleic acid amplification reaction.
Detachable diagnosis module supplies predetermined-temperature heating and detects amplification to determine diagnosis target
A diagnosis module main body detachably combined with the cartridge, the diagnosis module main body supplying heat at a predetermined temperature to the reaction chamber, detecting the nucleic acid amplification reaction, and determining whether or not a diagnosis target is present.
Cartridge plate architecture with tube accommodation, inlet port, outlet port, and fluid channel
A cartridge body formed in the shape of a plate having a front surface and a rear surface, wherein the cartridge body comprises a tube accommodation body formed on the front surface with an internal space in which the buffer tube is inserted; an inlet port formed in the rear surface; an outlet port formed in the front surface arranged between the inlet port and the reaction chamber; and a fluid channel formed in the rear surface transporting the sample solution from the inlet port to the outlet port.
Reaction chamber with pre-injected reagent and predetermined-temperature heat
The reaction chamber formed within the fluid channel in the rear surface of the cartridge body, the reaction chamber accommodating the sample solution, containing a pre-injected reagent, supplied with the heat at the predetermined temperature, and performing the nucleic acid amplification reaction.
Outlet-port plugging member allows air passage and blocks sample solution passage
An outlet-port plugging member inserted into the outlet port, the outlet-port plugging member allowing air to pass through and blocking passage of the sample solution.
Across the independent claim structure, the key inventive features combine buffer tube preparation of a nucleic-acid-containing sample solution, cartridge transport to a reaction chamber performing nucleic acid amplification, and a detachable diagnosis module that supplies predetermined-temperature heat, detects amplification, and determines whether a diagnosis target is present. The cartridge architecture further specifies inlet/outlet and fluid-channel layout with an outlet-port plugging member configured to allow air while blocking sample solution.
Stated Advantages
Documented Applications
No documented applications found
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