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Publication Number
US-12419239-B2
Publication Date
2025-09-23
Expiration Date
Abstract
A rice cultivar designated ‘Ozark’ is disclosed herein. The present invention provides seeds, plants, and plant parts derived from rice cultivar Ozark. Further, it provides methods for producing a rice plant by crossing Ozark with itself or another rice variety. The invention also encompasses any rice seeds, plants, and plant parts produced by the methods disclosed herein, including those in which additional traits have been transferred into Ozark through the introduction of a transgene or by breeding Ozark with another rice cultivar.
Core Innovation
The disclosure relates to a rice cultivar ‘Ozark’ (Oryza sativa) and to seed, plants, and plant parts that have ‘Ozark’ physiological and morphological characteristics. Representative seed of the cultivar ‘Ozark’ is deposited under National Center for Marine Algae and Microbiota International Depositary Authority Accession No. 202305004. The disclosure also describes ‘Ozark’ performance and characteristics across developmental origin and multiple locations, including grain yield, milling yield, plant height, lodging, and grain quality parameters such as kernel dimensions, apparent amylose content, and gelatinization temperature.
The disclosure addresses producing ‘Ozark’-derived plants by breeding and by tissue culture. It describes ‘Ozark’ selfing, backcrossing, and hybrid production, as well as crossing ‘Ozark’ with itself or another rice variety and crossing ‘Ozark’ to populations to produce progeny. Progeny can be identified using statistical significance (5%) and through marker, morphological, and filial relationship approaches to determine similarity to ‘Ozark’ under the same environmental conditions.
In addition to breeding, the disclosure describes introducing additional traits into ‘Ozark’ via gene-converted (‘Ozark’) and transgenic introduction. It describes transgenic rice traits including herbicide resistance, insect resistance, bacterial resistance, fungal resistance, viral resistance, modified fatty acid or carbohydrate metabolism, and male sterility. It also provides regenerable cells or protoplasts produced from ‘Ozark’ plants and plants regenerated from such tissue culture, while maintaining ‘Ozark’ physiological and morphological characteristics.
Claims Coverage
The partial content includes one independent claim covering a rice seed of the cultivar ‘Ozark’. The claim set further provides refinements that add inventive features concerning deposited seed identity, regeneration from regenerable cells or protoplasts, and genetic introduction of additional traits using backcrossing and transgenic transformation, including a quantified 5% backcross verification criterion.
Rice seed of cultivar ‘Ozark’ deposited sample
A rice seed of the cultivar ‘Ozark,’ where a representative sample of the seed has been deposited under National Center for Marine Algae and Microbiota International Depositary Authority Accession No. 202305004.
Backcrossing to retain ‘Ozark’ characteristics at a 5% significance level
A method introducing a desired trait into rice cultivar ‘Ozark’ by repeatedly backcrossing ‘Ozark’ with another rice line expressing the desired trait, selecting progeny plants expressing the desired trait, and continuing backcrossing at least three times to yield higher-generation backcross progeny that express the desired trait and retain essentially all ‘Ozark’ physiological and morphological characteristics under the same environmental conditions as determined at a 5% significance level.
Transgenic transformation providing specified traits
A method transforming a rice plant or its cells with a transgene that provides at least one specified trait selected from herbicide resistance, insect resistance, bacterial resistance, fungal resistance, viral resistance, modified fatty acid metabolism, modified carbohydrate metabolism, or male sterility.
Herbicide resistance limited to a selected herbicide group
A herbicide-resistant rice plant produced by the transgenic transformation method in which the gene provides resistance to a selected herbicide from a specified group of herbicides.
Regenerable tissue culture from ‘Ozark’ and regenerated plants with ‘Ozark’ characteristics
A tissue culture comprising regenerable cells or protoplasts produced from the rice plant of the ‘Ozark’ seed and a rice plant regenerated from the tissue culture that possesses the same ‘Ozark’ physiological and morphological characteristics.
Overall, the claim coverage anchors protection on deposited ‘Ozark’ seed and extends to ‘Ozark’-derived plants via regeneration from ‘Ozark’ tissue culture, via breeding/backcrossing with a quantified 5% similarity criterion, and via transgenic introduction of additional traits, including herbicide resistance defined by a selected group of herbicides.
Stated Advantages
Produces progeny plants that express a desired trait while retaining essentially all ‘Ozark’ physiological and morphological characteristics under the same environmental conditions at a 5% significance level.
Documented Applications
Producing ‘Ozark’-derived plants using selfing, crossing, backcrossing, and hybrid production to generate progeny from ‘Ozark’ seed.
Producing ‘Ozark’-derived plants by introducing additional traits into ‘Ozark’ through breeding (including repeatedly backcrossing) and by transgenic introduction.
Generating regenerable cells or protoplasts from ‘Ozark’ and regenerating plants from such tissue culture.
Producing herbicide-resistant rice plants with resistance to a selected herbicide from a specified group of herbicides.
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