Systems and methods for rapid nucleic acid extraction, purification and analysis from semen

Inventors

Selden, Richard F. • Turingan Witkowski, Rosemary

Assignees

Ande Corp

Abstract

Disclosed are processes and kits for rapid nucleic acid extraction from a nucleic acid-containing material, such as a bone, tooth or semen sample. For bone and tooth process involves providing the nucleic acid-containing material in a form suitable for nucleic acid extraction, adding a lysis buffer to the nucleic acid-containing material to obtain a mixture, mixing the mixture in a manner equivalent for about 30 seconds or longer and separating the mixture by centrifugation to obtain a liquid supernatant. The liquid supernatant contains the extracted nucleic acids which can be used for analysis including STR profiling by conventional or rapid DNA analysis. For semen the processes and kits involve applying an appropriate amount of sperm disruptive agent.

Core Innovation

The claimed process extracts nucleic acid from a semen sample collected on a swab and uses a sperm disruptive agent before direct transfer to a rapid DNA processing system without purification. The system obtains at least one STR profile from the sample. The claims identify neat semen or a semen stain as sample forms and specify interrogation of at least seven STR loci.

The description identifies neat semen and semen stains on substrates including denim and cotton as sample forms, with electropherograms displaying allele calls across multiple loci. Figures present STR profiles across loci including Amelogenin, D3S1358, D16S539, D2S441, D10S1248, D13S317, D5S818, TH01, vWA, D21S11, D18S51, DYS391, and SE33. Plotted signal peaks and labeled allele calls document STR profile generation across samples, alongside differential cell fraction separation.

Claims Coverage

The claims comprise one independent claim and two dependent claims covering three inventive features directed to rapid DNA processing of semen samples.

Independent claim 1 centers on direct processing of semen samples collected on a swab in a rapid DNA processing system without purification to obtain an STR profile, with dependent claims specifying sample forms and interrogation of at least seven STR loci.

Stated Advantages

Documented Applications