Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-12357761-B2
Publication Date
2025-07-15
Expiration Date
Abstract
A method of preparing a syringe in connection with a therapeutic treatment is disclosed. The method can include removing a plunger of the syringe from a barrel of the syringe, aligning the barrel in a horizontal orientation, filling a lumen of the barrel with a viscous material through an opening at a proximal end of the barrel, and inserting a plunger tip into the lumen to seal the lumen. The method can also include attaching an implantation device to a hub coupled to the barrel at a distal end of the barrel. The method can also include depressing the plunger until the cell suspension fills the implantation device and a droplet of the cell suspension is expelled from a distal tip of the implantation device.
Core Innovation
The invention relates to a method of treating a traumatic brain injury in a subject by administering a therapeutically effective amount of a cell suspension. The cell suspension comprises cells that are descendants from mesenchymal stem cells transiently-transfected by a polynucleotide encoding a Notch intracellular domain.
A syringe barrel with a proximal end, a distal end, and a lumen in between is used for preparation and delivery, and the plunger is removed and then reinserted after filling the lumen through an opening at the proximal end. The method couples an implantation device to the syringe at the distal end of the barrel and injects the cell suspension into the subject using the syringe and the implantation device at multiple deposit sites in the subject.
After each injection at a deposit site, at least one of the syringe and the implantation device is rotated about a longitudinal axis. The disclosure further describes delivering at multiple deposit sites using stereotactic guidance and administration trajectories, including retraction between sites and longitudinal rotation after injections, and implanting at brain/spinal/other CNS-related deposit sites.
Claims Coverage
The independent claim is directed to treating a traumatic brain injury using a specific cell suspension and a syringe/implantation-device injection workflow that includes multiple deposit sites and longitudinal rotation after each injection. The claim set includes one principal independent claim with dependent claims refining handling, dosing logistics, measurement, device structure, and spatial administration features.
Treatment of traumatic brain injury with transiently-transfected mesenchymal stem cell descendants
A method of treating a traumatic brain injury in a subject comprising filling a syringe lumen with a cell suspension having therapeutically effective amount of cells that are descendants from mesenchymal stem cells transiently-transfected by a polynucleotide encoding a Notch intracellular domain.
Filling by proximal opening after plunger removal and reinsertion
Removing a plunger of a syringe from a barrel of the syringe, wherein the barrel has a proximal end, a distal end, and a lumen in between; filling at least part of the lumen with the cell suspension through an opening at the proximal end; and reinserting the plunger.
Coupling to an implantation device and injecting at multiple deposit sites
Coupling an implantation device to the syringe at the distal end of the barrel; and injecting the cell suspension into the subject using the syringe and the implantation device at multiple deposit sites in the subject.
Longitudinal rotation about axis after each deposit-site injection
Rotating at least one of the syringe and the implantation device about a longitudinal axis after each injection at a deposit site of the multiple deposit sites.
Meniscus-based measurement of injected volume
Measuring the amount of a cell suspension injected by using a meniscus of the cell suspension remaining in the barrel.
Cannula hub and extension in fluid communication with syringe lumen
Defining the implantation device as a cannula with proximal and distal ends and a cannula hub having a cannula extension in fluid communication with the cannula lumen and extending into the lumen of a syringe at the syringe’s distal end.
Claim coverage focuses on a traumatic brain injury treatment method using transiently-transfected mesenchymal stem cell descendants, syringe lumen filling via plunger removal and proximal-end opening, injection via an implantation device at multiple deposit sites, and longitudinal rotation after each injection, with dependent refinements including meniscus-based measurement and a defined cannula-to-syringe fluid-communication configuration.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
Interested in licensing this patent?