Nanoparticles containing cellular membrane and uses thereof
Inventors
Gao, Weiwei • ZHU, HUIQING • Guan, Jian • Li, Yu-Wen
Assignees
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Publication Number
US-12350381-B2
Publication Date
2025-07-08
Expiration Date
Abstract
The present disclosure relates to nanoparticles containing cellular membrane and uses thereof. The nanoparticle comprises an interior compartment (or an inner core) and an outer surface (or shell) comprising a cellular membrane derived from a cell, said interior compartment (or an inner core) not providing a solid support to said cellular membrane in said outer surface (or shell). The present disclosure also relates to processes of making the nanoparticles. The present disclosure further relates to compositions comprising the nanoparticles and methods of using the nanoparticles.
Core Innovation
The invention relates to a nanoparticle comprising an interior compartment and an outer surface comprising a cellular membrane isolated from a cell. The interior compartment does not provide a solid support to the cellular membrane in the outer surface, and the interior compartment comprises a liquid that is isotonic to a human cellular or physiological liquid.
The outer surface comprises a steroid at a level from 40% (w/w) to 45% (w/w) and a sphingolipid at a level from 40% (w/w) to 50% (w/w), and from 10% (w/w) to 20% (w/w) of the cellular membrane isolated from the cell. The nanoparticle is prepared by an in vitro process.
The disclosure further emphasizes nanoparticle characteristics including cargo, particle size/shape, reduced source-cell constituents including hemoglobin, and reduced immunogenicity, along with therapeutic and immunogenic uses such as toxin/cytokine neutralization and disease treatment.
Claims Coverage
The partial content provides one independent claim. It specifies a nanoparticle architecture with a non-solid-support interior compartment, a cellular-membrane outer surface, and defined weight-percentage ranges for steroid and sphingolipid, and requires preparation by an in vitro process.
Liquid-filled interior compartment not providing solid support
An interior compartment and an outer surface comprising a cellular membrane isolated from a cell, wherein the interior compartment does not provide a solid support to the cellular membrane in the outer surface.
Isotonic liquid interior compartment
The interior compartment comprises a liquid that is isotonic to a human cellular or physiological liquid.
Steroid- and sphingolipid-enriched cellular membrane outer surface
The outer surface comprises a steroid at a level from 40% (w/w) to 45% (w/w) and a sphingolipid at a level from 40% (w/w) to 50% (w/w), and from 10% (w/w) to 20% (w/w) of the cellular membrane isolated from the cell.
In vitro process preparation
The nanoparticle is prepared by an in vitro process.
The inventive coverage combines a liquid-filled interior compartment that does not solidly support a cellular-membrane outer surface, an isotonic interior liquid, specified steroid and sphingolipid levels in the cellular membrane, and preparation by an in vitro process.
Stated Advantages
Reduced immunogenicity.
Reduced source-cell constituents including hemoglobin.
Documented Applications
Delivery and medicament/pharmaceutical compositions, including therapeutic and immunogenic applications such as toxin/cytokine neutralization and disease treatment.
Immunogenic composition comprising an effective amount of the nanoparticle.
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