Tuberculosis diagnosis method and apparatus therefor
Inventors
Choi, Dong Hwan • Kang, Kyungjoon • Lee, Won Hee
Assignees
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Publication Number
US-12306182-B2
Publication Date
2025-05-20
Expiration Date
Abstract
An apparatus for easily and conveniently diagnosing tuberculosis including a blood collecting device is provided. The blood collecting device comprises a support part including a first surface, a second surface formed opposite to the first surface, and a communication hole through which a solution is movable. The communication hole is formed between the first surface and the second surface. A blood collecting tip is attached to the first surface and has a capillary tube connected to the communication hole. According to the present disclosure, a small amount of peripheral blood is collected using a capillary phenomenon and the collected whole blood is used as it is without separating a blood corpuscle therefrom by centrifugation and the like to diagnose tuberculosis.
Core Innovation
The invention relates to a tuberculosis diagnosis apparatus that includes a support part with a communication hole and a dispensing nozzle, a blood collection tip having a capillary formed therein, and a reaction tube coupled to the support part and surrounding the blood collection tip so that an immune response takes place. The support part and communication hole allow a solution to move between a first side and a second side, while the reaction tube is arranged to receive the reacted material from the blood collection area.
The blood collection tip is configured to accept a rod-shaped part whose outer diameter is smaller than the inner diameter of the blood collection tip capillary, where the rod-shaped part has a Mycobacterium tuberculosis antigen immobilized. This rod-shaped, antigen-immobilized structure is inserted into the capillary of the blood collection tip, providing antigen-specific immune response in the reaction tube using a tuberculosis-specific antigen immobilized on the rod-shaped part.
In described embodiments, a rod-shaped part can provide immobilized antigen, including embodiments where the Mycobacterium tuberculosis antigen is in a dried state, and immune response detection is further supported by interferon gamma (IFN-b3) measurement components. An interferon gamma measurement cartridge can be included, and the cartridge can include a blood cell separation pad to reduce nonspecific reactions before detection at a T position and a C position associated with a fluorescence readout using antibody conjugates and a control conjugate.
Claims Coverage
The claim coverage identifies six inventive features centered on the capillary blood collection tip with antigen presentation via a rod, and IFN-b3 detection using labeled antibody conjugates and an interferon gamma measurement cartridge with a blood cell separation pad.
Capillary blood collection tip coupled to a reaction tube for immune response
A support part includes a first side, a second side opposite the first side having a dispensing nozzle, and a communication hole through which a solution can move between the first side and the second side; a blood collection tip attached to the first side and having a capillary formed therein; and a reaction tube coupled to the first side and surrounding the blood collection tip, wherein an immune response takes place.
Rod inserted into the blood collection tip capillary with Mycobacterium tuberculosis antigen immobilized
A rod-shaped part having an outer diameter smaller than an inner diameter of the blood collection tip, which is inserted into the capillary of the blood collection tip, and to which a Mycobacterium tuberculosis antigen is immobilized.
Dried Mycobacterium tuberculosis antigen for immune response
The Mycobacterium tuberculosis antigen is provided in a dried state.
Interferon gamma antibody conjugates and control conjugate in a buffer tube
A buffer tube contains antibody conjugates specific for interferon gamma and a buffer.
Interferon gamma measurement cartridge
An interferon gamma measurement cartridge is included.
Blood cell separation pad within the interferon gamma measurement cartridge
The interferon gamma measurement cartridge includes a blood cell separation pad.
Overall, the claim set covers a tuberculosis diagnosis apparatus that creates an immune response in a reaction tube using a capillary-type blood collection tip with an inserted rod carrying a Mycobacterium tuberculosis antigen immobilized, with dependent features refining antigen presentation and adding interferon gamma detection via labeled antibody conjugates in a buffer and an interferon gamma measurement cartridge that includes a blood cell separation pad.
Stated Advantages
Simpler, quicker TB/latent TB testing from peripheral blood with a whole blood workflow suitable to avoid complex equipment and procedures.
Documented Applications
TB/latent TB testing using peripheral blood with a simplified whole blood workflow.
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