Detection method
Inventors
PEDRO SANTOS MANSO CÔRTE-REAL, José • MIGUEL SILVA DO CARMO NOGUEIRA, José • MIGUEL ALVES FARINHA, André • EDUARDO ARTILHEIRO FERREIRA, Alberto • FILIPE PINTO SILVA, Tiago • MIGUEL MELO RIBEIRO, Mauro • Da Conceição Fernandes Thomaz, Filipe • MANUEL DE OLIVEIRA GARCIA DA FONSECA, João
Assignees
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Publication Number
US-12298230-B2
Publication Date
2025-05-13
Expiration Date
Abstract
A detection system including a detection device and an assay device. The detection device includes a space for receiving an assay device, a drive arrangement engaging the assay device to rotate the assay device, a light source arranged to direct light towards the assay device, and a diffuser arranged to diffuse light from the light source. The diffuser is arranged between the light source and the space. The detection device includes a light receiver arranged to receive light from the light source that has passed through the assay device.
Core Innovation
The invention provides a method of determining an effect of a liquid sample on light by rotating an assay device comprising one or more chambers for containing the liquid sample. Light is directed from a light source to the rotating assay device such that the light passes through the one or more chambers as each chamber is in a respective predetermined configuration relative to the light source and a light receiver, and the light source and the light receiver are arranged on the same side of the rotating assay device.
The method receives, at the light receiver, light that has passed through the one or more chambers for at least one complete rotation of the assay device, and determines a set of light intensity measurements for each complete rotation. The method determines measurements of light intensity within each set obtained when the light passed through the one or more chambers and determines the number of measurements that meet a light intensity condition in one of the sets.
For subsequent complete rotations, the method changes the light intensity condition based on the number of measurements that met the light intensity condition. Using the measurements of light intensity obtained when the light passed through the one or more chambers, the method determines the effect of the liquid sample on the light from the light source. A detection system includes an assay device with one or more chambers, a drive arrangement for rotating the assay device, one or more light sources and corresponding light receivers, and one or more processors programmed to perform these steps.
Claims Coverage
The document includes two independent claims that share a common core: rotating an assay device with one or more chambers, measuring transmitted light intensity over complete rotations, and adaptively changing a light intensity condition based on the number of measurements meeting that condition in order to determine an effect of the liquid sample on the light. The coverage also includes reflective optical routing, quality-control rejection of measurement sets based on chamber count and chamber position relative to a reference device, optional memory storage of non-rejected measurement sets, and optional rotation speed adjustment based on the number of measurements in a set.
Same-side light source and light receiver with predetermined chamber configuration
Directing light from a light source to the rotating assay device such that the light passes through the one or more chambers as each chamber is in a respective predetermined configuration relative to the light source and a light receiver, and wherein the light source and the light receiver are arranged on the same side of the rotating assay device.
Sets of light intensity measurements per complete rotation and chamber-passed measurements
Receiving, at the light receiver, light from the light source that has passed through the one or more chambers within the rotating assay device for at least one complete rotation; determining a set of light intensity measurements for each complete rotation; determining measurements of light intensity within each set that were obtained when the light passed through the one or more chambers.
Adaptive change of light intensity condition based on number of measurements meeting the condition
Determining the number of measurements that meet a light intensity condition in one of the sets of measurements; changing the light intensity condition for sets of measurements for subsequent complete rotations based on the number of measurements that met the light intensity condition.
Effect determination using measured light intensity passed through chambers
Determining, using the measurements of light intensity that were obtained when the light passed through the one or more chambers, the effect of the liquid sample on the light from the light source.
Reflective optical routing for directing light to and from chambers
Directing the light from the one or more light sources towards a corresponding one of the one or more chambers within the assay device and receiving light at a corresponding light receiver from the one or more chambers, wherein the system uses first and second reflecting surfaces to direct light between the light source, the one or more chambers, and the light receiver.
Rejecting measurement sets based on chamber count versus expected number
Determining the number of chambers in each set of light intensity measurements and rejecting the respective set of measurements when the number of chambers is not as expected.
Rejecting measurement sets based on chamber position versus reference device
Determining the position of a reference device and, for each set of measurements, rejecting the respective set of measurements when the one or more chambers are not in an expected position relative to the reference device.
Storing non-rejected measurement sets in memory
Using one or more processors and memory to store in memory the sets of measurements of light intensity that are not rejected.
Changing rotation speed based on number of measurements in a set
Determining how many measurements are in a set and changing the speed of rotation of the assay device based on that determined number of measurements.
Overall, the claim coverage centers on adaptive determination of a liquid-sample effect on light using intensity measurements collected over complete rotations of a rotating assay device, with same-side optical arrangement, reflective routing, quality-control rejection using chamber count and chamber position checks, optional storage of accepted data, and rotation speed adjustment.
Stated Advantages
Documented Applications
No documented applications found
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