Electrochemical sensor for analysis of beverages
Inventors
TEO, Stanley Zhen-Jin • Newman, Peter Michael • Chatelier, Ronald Christopher • COSSINS, Luke Robert • Hodges, Alastair M.
Assignees
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Publication Number
US-12292405-B2
Publication Date
2025-05-06
Expiration Date
Abstract
The invention disclosed herein relates to a dry sensor for measuring the concentration of an analyte in a liquid beverage sample. Described herein is a novel dry sensor which is able to receive a liquid sample and adjust the pH of the liquid to be suitable for assaying an analyte of interest without the need to add reagents to the sample and/or to perform manually timed operations and able to detect a redox reaction in the presence of a liquid sample. The meter disclosed herein, when connected to the sensor disclosed herein is able to adjust the temperature of the liquid to be suitable for the assay, apply a series of potentials, measure the current at several times, measure the diffusion coefficient of the limiting electrochemical species, calculate the concentration of one or more analytes, and rapidly provide the user with the required information on the liquid sample.
Core Innovation
The invention provides a sensor for assessing an analyte of interest in a liquid beverage sample. The sensor includes a first electrode, an opposing surface to the first electrode, and a second electrode, where the opposing surface comprises the second electrode or is a plain opposing surface. The first electrode is coated with a reagent that includes an electroactive species, and the sensor is configured to detect a redox reaction in the presence of the liquid beverage sample.
The opposing surface is coated with a pH modifying reagent capable of adjusting the pH of the liquid sample to be suitable for assaying the analyte of interest. The pH modifying reagent comprises a mixture of citraconic acid and oxalic acid. By adjusting the sample pH at the sensor surface, the system enables the assay to proceed using the electroactive species on the first electrode.
The disclosed system is implemented as a dry electrochemical beverage sensor strip with opposing or coplanar electrodes, including examples of electrode/substrate construction and strip architecture for a single-use chamber/channel configuration. The sensor applies electrochemical potentials/currents and measures current versus time, including background subtraction and determining the diffusion coefficient of a limiting electrochemical species, which is used to calculate analyte concentration. Example analytes explicitly described include free sulphur dioxide (SO2), total sugar, glucose, fructose, and malic acid.
Claims Coverage
The patent contains one independent claim directed to a dry electrochemical sensor architecture for beverage analytes. Dependent claim coverage refines the independent claim by specifying analytes, pH modifying reagent details, electroactive species options, electrode geometry, and optional additional enzyme coating and pH range constraints.
Beverage analyte sensor with opposing electrodes and redox detection
A sensor for assessing an analyte of interest in a liquid beverage sample using a first electrode, an opposing surface to the first electrode, and a second electrode or a plain opposing surface, with the first electrode coated with a reagent including an electroactive species and the sensor configured to detect a redox reaction in the presence of the liquid beverage sample.
Opposing surface pH modification using citraconic acid and oxalic acid mixture
The opposing surface is coated with a pH modifying reagent capable of adjusting the pH of the liquid sample to be suitable for assaying the analyte of interest, wherein the pH modifying reagent comprises a mixture of citraconic acid and oxalic acid.
Analyte selection for beverage assessment
The sensor is configured to analyze an analyte of interest selected from free sulphur dioxide, total sugar, glucose, fructose, or malic acid.
Electroactive species options for the first electrode reagent
The reagent on the first electrode includes an electroactive species selected from nicotinamide adenine dinucleotide, potassium ferricyanide, ferric ethylenediaminetetraacetic acid, phenazine ethosulfate, 1,2-napthoquinone-4-sulfonic acid, or combinations/mixtures thereof.
Electrode-facing geometry
The sensor includes first and second electrodes that face each other.
Optional glucose-selective enzyme on electrode or opposing surface
A first electrode or opposing surface is further coated with a glucose-selective enzyme.
Explicit pH range constraints for the pH modifying reagent
The pH modifying reagent adjusts the liquid sample to one of several specified pH ranges of 1–3, 4–5, or 6–9.
Coverage centers on a beverage analyte electrochemical redox sensor that uses a first electrode reagent containing an electroactive species and a pH-modifying opposing surface coated with a citraconic acid and oxalic acid mixture, with further refinements specifying analyte sets, electroactive species alternatives, electrode-facing geometry, optional glucose-selective enzyme coating, and explicit pH range constraints.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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