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Publication Number
US-12290612-B2
Publication Date
2025-05-06
Expiration Date
Abstract
A scaffold comprising an aligned fiber. Further, a scaffold comprising one or more electrospun fibers wherein a fast Fourier transform (FFT) analysis result of the fibers have adjacent major peaks with about 180° apart from each other. Also, methods for promoting differentiation of stem cells into osteoblasts, chondrocytes, ligament or tendon, the method comprising culturing the cells on the scaffold or aligned fiber in conditions suitable for the cell differentiation.
Core Innovation
The invention relates to a composite scaffold comprising multiple layers of sheets. At least a first layer and a second layer are included, where the first layer comprises electrospun fibers comprising collagen and a copolymer. The first-layer fibers are arranged such that a result of a fast Fourier transform (FFT) analysis demonstrates that the fibers are aligned, with major adjacent peaks about 180° apart.
The composite scaffold includes a second layer comprising random fibers. The first layer provides aligned electrospun collagen/copolymer fibers as determined by FFT, while the second layer provides random fibers as a contrasting layer within the multi-layer sheet scaffold.
The disclosed scaffold is used for culturing stem cells to promote lineage differentiation, including osteoblasts, chondrocytes, and ligament/tendon differentiation. The scaffold is also used for nerve repair by supporting dorsal root ganglia attachment and neurite outgrowth, as documented by examples using basement membrane extract and a copolymer scaffold format.
Claims Coverage
The relevant independent claim is one: clm-00001. It contains multiple inventive features, primarily the multi-layer sheet architecture with an aligned collagen/copolymer electrospun first layer characterized by FFT peak spacing, and a second random-fiber layer.
Multilayer sheet composite scaffold with aligned first layer and random second layer
A composite scaffold comprising multiple layers of sheets including at least a first layer and a second layer, wherein the first layer comprises electrospun fibers comprising collagen and a copolymer, and the second layer comprises random fibers.
FFT-verified fiber alignment with major adjacent peaks about 180° apart
The first layer fibers are aligned as demonstrated by a result of a fast Fourier transform (FFT) analysis, showing major adjacent peaks that are about 180° apart from each other.
Electrospun collagen/copolymer first-layer fibers
The first layer comprises electrospun fibers comprising collagen and a copolymer.
Across the independent claim, the inventive concept is a composite scaffold with layered sheets in which an electrospun collagen/copolymer first layer exhibits FFT-indicated alignment (major adjacent peaks about 180° apart) while a second sheet layer comprises random fibers.
Stated Advantages
Aligned electrospun collagen/copolymer fibers are achieved and quantified by FFT showing major adjacent peaks about 180° apart.
Scaffolds support dorsal root ganglia attachment and outgrowth in documented examples.
Documented Applications
Culturing stem cells to promote lineage differentiation, including osteoblasts, chondrocytes, and ligament/tendon differentiation.
Nerve repair by supporting dorsal root ganglia attachment and neurite outgrowth, including documented basement membrane extract/copolymer scaffold examples.
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