Agricultural compositions comprising remodeled nitrogen fixing microbes
Inventors
REISINGER, MARK • SANDERS, ERNEST • BROGLIE, RICHARD • Temme, Karsten
Assignees
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Publication Number
US-12290074-B2
Publication Date
2025-05-06
Expiration Date
Abstract
The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
Core Innovation
A composition is provided comprising a plurality of non-intergeneric remodeled bacteria and at least one fertilizer. The non-intergeneric remodeled bacteria produce fixed N at at least about 1×10^-17 mmol N per bacterial cell per hour and have an average colonization ability per unit of plant root tissue of at least about 1.0×10^4 bacterial cells per gram of fresh weight of plant root tissue.
The fertilizer comprises ammonia, glutamine, urea, and a nitrate, or one or more of nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, boron, chloride, manganese, iron, zinc, copper, molybdenum, selenium, and any salt thereof. Alternatively, the fertilizer comprises one or more of an amino acid, a salt, a vitamin, glucose, sodium chloride, a yeast extract, ammonium dihydrogen phosphate, ammonium sulfate, glycerol, lactic acid, propionic acid, succinic acid, malic acid, citric acid, potassium bitartrate, xylose, lyxose, and lecithin, or one or more of a nitrification inhibitor, a nitrogen stabilizer, and a urease inhibitor.
The disclosure also addresses applying a composition comprising a plurality of non-intergeneric remodeled bacteria and at least one fertilizer to a plant or to a field that will comprise a plant. The same stated performance thresholds are maintained for fixed N production and average colonization ability on plant root tissue, and the composition can be pre-combined before application.
Claims Coverage
Two independent claims are present: a composition claim and a method of applying the composition to a plant or field. Each independent claim includes three core inventive features: non-intergeneric remodeled bacteria with fixed N production above a stated threshold, non-intergeneric remodeled bacteria with root colonization ability above a stated threshold, and at least one fertilizer selected from enumerated categories.
Composition with non-intergeneric remodeled bacteria and fertilizer
A composition comprising a plurality of non-intergeneric remodeled bacteria and at least one fertilizer, wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10^-17 mmol N per bacterial cell per hour and have an average colonization ability per unit of plant root tissue of at least about 1.0×10^4 bacterial cells per gram of fresh weight of plant root tissue.
Fertilizer composition alternatives
The at least one fertilizer comprises one or more of ammonia, glutamine, urea, and a nitrate; or one or more of nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, boron, chloride, manganese, iron, zinc, copper, molybdenum, selenium, and any salt thereof; or one or more of an amino acid, a salt, a vitamin, glucose, sodium chloride, a yeast extract, ammonium dihydrogen phosphate, ammonium sulfate, glycerol, lactic acid, propionic acid, succinic acid, malic acid, citric acid, potassium bitartrate, xylose, lyxose, and lecithin; or one or more of a nitrification inhibitor, a nitrogen stabilizer, and a urease inhibitor.
Applying the bacteria-fertilizer composition to a plant or field
A method of applying a composition comprising a plurality of non-intergeneric remodeled bacteria and at least one fertilizer to a plant or a field that will comprise a plant, wherein the non-intergeneric remodeled bacteria produce fixed N of at least about 1×10^-17 mmol N per bacterial cell per hour and have an average colonization ability per unit of plant root tissue of at least about 1.0×10^4 bacterial cells per gram of fresh weight of plant root tissue.
Across the independent claims, the coverage centers on non-intergeneric remodeled bacteria configured to produce fixed N at a stated minimum rate and to colonize plant root tissue at a stated minimum level, combined with an enumerated set of fertilizer types.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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