Transgenic betta

Inventors

Blake, Alan • Crockett, Richard • Nasevicius, Aidas

Assignees

Glofish LLC

Abstract

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Core Innovation

The invention relates to transgenic Betta fish that produce stable fluorescent coloration by having a chromosomally integrated expression cassette encoding a green, red or orange fluorescent protein. The transgenic Betta is defined by a specific transformation event corresponding to a Green Betta 1 transformation event, an Orange Betta 1 transformation event, or a Red Betta 1 transformation event, identified through corresponding ATCC-deposited sperm deposit numbers.

The document further describes chromosomally integrated transformation events for ornamental transgenic Betta, including Green Betta 1, Orange Betta 1, and Red Betta 1 transformation events, with stable fluorescent coloration. The narrative context also references fluorescent proteins in the set of green, orange, red, purple, and blue colors, including GFP, YFP, RFP, PFP, and BFP.

The described approach includes the use of cryopreserved testes and germ cells and deposited lines associated with the named transformation events, and provides for production and distribution of transgenic ornamental fish. The background describes fluorescence-based transgenesis and line regeneration, including regeneration from cryopreserved testes using germ cell implantation into fry, with visual inspection for fluorescence.

Claims Coverage

The document provides two independent claims: one directed to transgenic Betta with chromosomally integrated fluorescent-protein expression cassettes defined by specific Green, Orange, and Red Betta 1 transformation events and ATCC sperm deposit numbers, and one directed to cell lines containing those transformation events as identified by ATCC deposit numbers.

Overall claim coverage ties transgenic ornamental Betta to chromosomally integrated fluorescent-protein expression cassettes and to specifically identified Green Betta 1, Orange Betta 1, and Red Betta 1 transformation events using ATCC deposit-number identification, and it also covers cell lines containing the same transformation events for making such transgenic Betta.

Stated Advantages

Documented Applications