Genome extraction device of dual chamber structure in which outer chamber and inner chamber are combined with each other
Inventors
Cho, Young-Shik • Park, Hae-Joon • Lee, Sunyoung • LIM, Kwanhun • KIM, In-Ae • Kim, Dong-hun
Assignees
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Publication Number
US-12239979-B2
Publication Date
2025-03-04
Expiration Date
Abstract
Provided is a genome extraction device to which a dual chamber structure of an outer chamber and an inner chamber is applied, more particularly a genome extraction device to which the above-described dual chamber structure is applied so that the genome extraction device can be stored stably for a long time without the risk of reagent leakage.
Core Innovation
The invention relates to a specimen preparation component of a genome extraction device in which an outer chamber is partitioned into a plurality of first spaces by an outer chamber partition wall, and an inner chamber is coupled to the outer chamber through upper openings of the plurality of first spaces and is partitioned into a plurality of second spaces by an inner chamber partition wall. The inner chamber includes a first position and a second position for transferring a reagent accommodated in the second spaces to the first spaces.
The inner chamber has an upper inner chamber that is in close contact with an inner wall of the plurality of first spaces of the outer chamber, while a lower inner chamber is connected to the upper inner chamber and includes a curved portion toward a radially inner side. The curved portion is spaced apart from the inner wall of the plurality of first spaces, thereby addressing micro-leakage and cross-contamination via capillary action between inner and outer regions.
Fluid introduction between the inner chamber second spaces and the outer chamber first spaces is enabled by sealing members covering lower openings of the plurality of second spaces. In the second position, a protrusion member formed on a bottom surface of the plurality of first spaces tears open the sealing member to introduce the fluid accommodated in the plurality of second spaces into the plurality of first spaces. Sealing members are described as films in certain embodiments, and a safety clip is described to prevent sealing member perforation during vibration.
The specimen preparation component further includes piston structures and a flow-path sealed cover structure, and an amplification module. A piston includes a close contact portion and fluid accommodating portion with liquid port and filter port to inhale and discharge fluids into selected first spaces. An amplification preparation module coupled via injection port(s) includes a gas moving passage and an extract moving passage connecting the injection port to an accommodating portion, including passage geometry/length differences intended to manage air bubble removal and prevent mixing between multiple accommodating portions.
Claims Coverage
The document provides one independent claim (clm-00001) for a specimen preparation component. The claim contains two core inventive features: the dual-chamber partitioned structure with an upper close-contact region and a lower curved spacer region, and a sealing-member tearing mechanism that introduces reagent fluid from the inner chamber second spaces into the outer chamber first spaces when the inner chamber is in the second position. Dependent claims refine sealing-member form and add piston/port and amplification-module passage features.
Dual-chamber partitioned reagent introduction structure with upper close contact and lower curved spacing
An outer chamber partitioned into a plurality of first spaces by an outer chamber partition wall, and an inner chamber coupled through upper openings and partitioned into a plurality of second spaces by an inner chamber partition wall, where the inner chamber includes an upper inner chamber in close contact with an inner wall of the plurality of first spaces and a lower inner chamber connected to the upper inner chamber and including a curved portion toward a radially inner side so as to be spaced apart from the inner wall of the plurality of first spaces.
Second-position sealing member tearing by a protrusion member to introduce inner fluid to outer first spaces
Lower openings of the plurality of second spaces of the inner chamber are covered by a second sealing member, and in the second position a second protrusion member formed on a bottom surface of the plurality of first spaces tears open the second sealing member allowing fluid accommodated in the plurality of second spaces to be introduced into the first spaces of the outer chamber.
Overall, the claim coverage centers on a dual-chamber, partitioned genome extraction specimen-preparation component with an upper inner chamber in close contact with the outer chamber inner wall and a lower inner chamber having a curved portion spaced from that wall, together with a sealing-member tearing mechanism in the second position that transfers fluid from inner second spaces to outer first spaces. Dependent coverage further specifies sealing members as films and adds piston/port inhaling-discharge behavior and an amplification preparation module with injection-related gas and extract moving passages.
Stated Advantages
Prevents micro-leakage and cross-contamination via capillary action between inner and outer regions.
Sealing-member tearing enables controlled introduction of fluid from the inner chamber second spaces into the outer chamber first spaces.
A safety clip is used to prevent sealing member perforation during vibration.
Passage geometry/length differences in the amplification module are intended to manage air bubble removal and prevent mixing between multiple accommodating portions.
Documented Applications
Specimen preparation component of a genome extraction device for using a reagent for analysis of a specimen.
Amplification preparation module coupled to the genome extraction device via injection port(s) using an accommodating portion with gas moving passage and extract moving passage.
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