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Publication Number
US-12225888-B2
Publication Date
2025-02-18
Expiration Date
Abstract
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by m vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Core Innovation
The invention concerns transgenic rainbow shark sperm whose genome comprises exogenous nucleic sequence(s) encoding foreign fluorescent protein(s). The transgenic rainbow shark sperm is identified by ECACC depositions as specific Accession Numbers: 19010802 for an orange fluorescent protein, 19010801 for a purple fluorescent protein, 19010803 for a blue fluorescent protein, and 19010804 for a green fluorescent protein. The exogenous nucleic sequences encode the fluorescent proteins as part of the sperm genome.
The disclosed system addresses the problem of providing transgenic rainbow shark lines with defined fluorescent protein expression, specifically covering orange, purple, blue, and green fluorescent proteins. The document frames the transgenic outcome as producing a transgenic rainbow shark that functionally expresses the corresponding fluorescent protein(s) from the foreign gene(s). The background and motivation establish the need for such fluorescent transgenic rainbow sharks using foreign fluorescent-protein genes.
The description further provides the use of the deposited transgenic rainbow shark sperm in producing transgenic offspring and positioning them for downstream use. It describes fertility and inclusion of eggs, sperm, embryos, progeny, and outlines provisioning/distribution activity associated with supply for the ornamental fish market. The disclosed lines are associated with visualization under white/sunlight/UV/blue light as part of the intended functional outcome.
Claims Coverage
The independent claim provides a deposit-identified transgenic rainbow shark sperm defining exogenous nucleic sequences encoding orange, purple, blue, or green fluorescent proteins, each tied to a specific ECACC Accession Number. Dependent claims then refine production of transgenic rainbow sharks by fertilization and constrain resulting characteristics and downstream provisioning for an ornamental fish market.
Deposited transgenic rainbow shark sperm with foreign fluorescent protein-encoding exogenous nucleic sequences
A transgenic rainbow shark sperm whose genome comprises an exogenous nucleic sequence encoding an orange fluorescent protein deposited at the ECACC as Accession Number 19010802; an exogenous nucleic sequence encoding a purple fluorescent protein deposited at the ECACC as Accession Number 19010801; an exogenous nucleic sequence encoding a blue fluorescent protein deposited at the ECACC as Accession Number 19010803; or an exogenous nucleic sequence encoding a green fluorescent protein deposited at the ECACC as Accession Number 19010804.
Producing a transgenic rainbow shark via fertilization to functionally express fluorescent proteins
A method of making a transgenic rainbow shark by fertilizing a rainbow shark egg with sperm of the ECACC-deposited transgenic sperm, to obtain a transgenic shark that functionally expresses an orange, purple, blue, or green fluorescent protein.
Using non-transgenic rainbow shark eggs for the fertilization method
The method uses rainbow shark eggs that are non-transgenic.
Fertile transgenic rainbow shark expressing fluorescent protein
The transgenic rainbow shark is fertile.
Providing and distributing transgenic rainbow sharks to the ornamental fish market
A method of providing a transgenic rainbow shark to the ornamental fish market, comprising producing a transgenic rainbow shark using the fertilization method and distributing the fish to the ornamental fish market.
Ornamental fish market retailer as a multi-product retailer
The retailer is a multi-product retailer having an ornamental fish department.
Overall claim coverage centers on ECACC-deposited transgenic rainbow shark sperm with exogenous nucleic sequences encoding orange, purple, blue, or green fluorescent proteins, followed by producing transgenic sharks through fertilization of rainbow shark eggs that functionally express the fluorescent proteins. Additional dependent coverage includes fertility and downstream provisioning/distribution to the ornamental fish market, including restriction of the retailer to a multi-product retailer with an ornamental fish department.
Stated Advantages
Produces transgenic rainbow shark offspring that functionally express fluorescent proteins (orange, purple, blue, or green).
Supports visualization under white/sunlight/UV/blue light.
Provides transgenic rainbow sharks for the ornamental fish market, including provisioning/distribution.
Documented Applications
Provisioning and distribution of transgenic rainbow sharks to the ornamental fish market.
Use as visualization/utility tools associated with fluorescence under white/sunlight/UV/blue light.
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