Swine origin probiotics that promote health and growth performance in pigs
Inventors
Zhao, Jiangchao • Wang, Xiaofan • Tsai, Tsungcheng • Maxwell, Charles V.
Assignees
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Publication Number
US-12220435-B2
Publication Date
2025-02-11
Expiration Date
Abstract
The present invention provides probiotic compositions comprising isolated bacterial strains, referred to herein as LactX, StrepX1 and StrepX3. Methods of using the compositions to increase growth rate in pigs and enhance pig production are also provided.
Core Innovation
The invention relates to swine probiotic compositions comprising isolated bacterial strains LactX (Lactobacillus) and StrepX1/StrepX3 (Streptococcus) that are selected for growth-performance association during longitudinal gut microbiome profiling from birth to market. The compositions are provided as dried animal feed compositions containing lyophilized isolated bacterial strains, including LactX, with strain identity supported by ATCC deposit accessions.
The disclosed compositions are used for pigs by administering the probiotic compositions to increase growth rate and mitigate the negative impacts associated with weaning. The invention further characterizes the disclosed bacterial strains using 16S rRNA V4 features and includes ATCC deposit information for the isolates.
The document includes characterization and microbiome profiling results, including fecal microbiota transplantation (FMT) showing increased growth performance without markedly altering overall microbiome structure, and testing indicating that StrepX3 and LactX can increase body weight versus control. Formulations described include lyophilized/dried powder and feed incorporation, including direct-fed microbial (DFM) compositions containing the disclosed strains.
Claims Coverage
The independent claim defines a dried animal feed composition containing a lyophilized isolated Lactobacillus strain LactX deposited at ATCC under accession number PTA-127487, and requires a minimum level of LactX colony forming units per serving. The claim set further identifies Streptococcus strains StrepX3 or StrepX1 with specified 16S rRNA sequence information and ATCC accession numbers, and includes direct-fed microbial compositions.
ATCC-deposited Lactobacillus strain LactX in dried animal feed
A composition comprising a dried animal feed and a lyophilized isolated bacterial strain comprising Lactobacillus strain LactX, wherein LactX is deposited at the American Type Culture Collection (ATCC) under accession number PTA-127487.
Minimum LactX colony forming units per serving
The composition contains at least 10^8 colony forming units of LactX per serving.
Lyophilized Streptococcus strains StrepX3 or StrepX1 with defined 16S rRNA sequence
A composition containing an additional lyophilized isolated bacterial strain comprising Streptococcus strains StrepX3 or StrepX1, where StrepX3 and StrepX1 have a specified 16S rRNA sequence (SEQ ID NO:2).
ATCC-deposited StrepX1 and StrepX3 accessions
The composition defines StrepX1 and StrepX3 as specific bacterium strains deposited at the ATCC with accession numbers PTA-127485 and PTA-127486, respectively.
Direct-fed microbial composition
The composition is a direct-fed microbial composition.
Overall, the claims cover specific lyophilized probiotic bacterial strains identified by ATCC deposit accessions and, for Streptococcus, by specified 16S rRNA sequence information, formulated in dried animal feed and limited to compositions containing at least 10^8 LactX colony forming units per serving, including embodiments characterized as direct-fed microbial compositions.
Stated Advantages
Increase growth rate and growth performance in pigs.
Alleviate detrimental effects of weaning.
Reduce time from birth to marketable weight.
Increased growth performance in fecal microbiota transplantation (FMT) without markedly altering overall microbiome structure.
StrepX3 and LactX increased body weight versus control when tested at weaning.
Example 2 indicates that an ADG improvement can be comparable to antibiotics.
No detrimental carcass quality impact in the disclosed comparison.
Documented Applications
Administering the probiotic compositions to pigs to increase growth rate.
Administering the probiotic compositions to alleviate detrimental effects of weaning.
Administering the probiotic compositions to reduce time from birth to marketable weight.
Use of fecal microbiota transplantation (FMT) associated with increased growth performance without markedly altering overall microbiome structure (as documented in the study results).
Testing showing increased body weight versus control when StrepX3 and LactX are used in vivo at weaning.
Comparative assessment in Example 2 of growth performance (ADG) versus antibiotics, including an indication of no detrimental carcass quality impact.
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