Red transgenic fluorescent ornamental fish
Inventors
Blake, Alan • Crockett, Richard • Nasevicius, Aidas
Assignees
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Publication Number
US-12213465-B2
Publication Date
2025-02-04
Expiration Date
Abstract
The present invention relates to transgenic red ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Core Innovation
The invention relates to transgenic barb progeny whose genome is obtained from a first transgenic barb that exhibits fluorescence. The first transgenic barb comprises a chromosomally integrated expression cassette encoding a fluorescent protein, and the chromosomally integrated expression cassette is comprised by the "Red barb 1 transformation event." The transgenic barb progeny exhibits fluorescence.
The invention further relates to linking the chromosomally integrated cassette in the first transgenic barb to sperm deposited having the "Red barb 1 transformation event." In particular, the sperm comprising the "Red barb 1 transformation event" is deposited as ECACC accession no. 16121901.
The disclosed system is extended through progeny generation in which the transgenic barb progeny is obtained from the fluorescent first transgenic barb. In additional refinements associated with the progeny, the first and/or second barb may be specified as heterozygous or homozygous for the "Red barb 1 transformation event," and the second barb may be required to be fluorescent as well.
Claims Coverage
The document includes one independent claim defining fluorescent transgenic barb progeny derived from a first fluorescent transgenic barb containing a chromosomally integrated fluorescent-protein expression cassette comprised by the "Red barb 1 transformation event," with event traceability to ECACC accession no. 16121901; the claim requires that the progeny exhibits fluorescence. Dependent claims refine zygosity and further specify fluorescence of the second barb and an additional ECACC traceability detail for a fluorescent-protein cassette.
Fluorescent transgenic barb progeny from Red barb 1 event
A transgenic barb progeny whose genome is obtained from a first transgenic barb, wherein the first transgenic barb exhibits fluorescence and comprises a chromosomally integrated expression cassette encoding a fluorescent protein, and wherein the chromosomally integrated expression cassette in the first transgenic barb is comprised by the "Red barb 1 transformation event," and the transgenic barb progeny exhibits fluorescence.
Event traceability via ECACC-deposited Red barb 1 sperm
Sperm comprising the "Red barb 1 transformation event" having been deposited as ECACC accession no. 16121901, such that the transgenic barb progeny exhibits fluorescence.
Zygosity selection for the first barb Red barb 1 event
The transgenic barb progeny wherein the first barb is heterozygous for the "Red barb 1 transformation event" or wherein the first barb is homozygous for the "Red barb 1 transformation event".
Fluorescent second barb with Red barb 1 chromosomal cassette
The transgenic barb progeny wherein the second barb is a transgenic barb that exhibits fluorescence, and wherein the second barb contains a chromosomally integrated expression cassette for a fluorescent protein from the "Red barb 1 transformation event" using sperm from ECACC accession no. 1612901.
Zygosity selection for the second barb Red barb 1 event
The transgenic barb progeny wherein the second barb is homozygous for the "Red barb 1 transformation event".
Overall, claim coverage centers on fluorescent transgenic barb progeny derived from a first fluorescent transgenic barb with a chromosomally integrated fluorescent-protein expression cassette comprised by the "Red barb 1 transformation event," with ECACC accession traceability for the deposited sperm material, and with dependent refinements specifying zygosity and fluorescence of the relevant bars and cassette correspondence.
Stated Advantages
Documented Applications
No documented applications found
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