Digital mirror device based code-division multiplexed Raman optical mapping system for wide field imaging
Inventors
Kersey, Alan • Pandey, Rishikesh
Assignees
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Publication Number
US-12163891-B2
Publication Date
2024-12-10
Expiration Date
Abstract
A system and method for mapping a tissue sample is provided. The system includes a light source, a scanner, a digital mirror device (DMD), a light detector, and an analyzer. The DMD has an array of micromirrors. The analyzer controls the light source, controls the scanner, controls the DMD to have on-state micromirrors aligned with a light beam, and other micromirrors in an off-state. The on-state micromirrors direct the light beam to a tissue sample. The analyzer assigns one or more location codes to the on-state micromirrors, controls the light detector to receive Raman light emitted from the tissue sample, correlates the location codes of the on-state micromirrors with light detector signals representative of the Raman emitted light, and produces a spatial map of the Raman emitted light.
Core Innovation
The invention provides a system for mapping a tissue sample by using a digital mirror device (DMD) with an orthogonal array of micromirrors that are selectively controllable to an on-state or an off-state. A light source emits a beam of light, and a scanner scans the DMD with the beam of light. The system controls a subset of micromirrors in the on-state aligned with the beam of light to direct the beam to a tissue sample location, while micromirrors outside the subset are in the off-state.
For spatial localization, the system assigns one or more location codes to the subset of micromirrors in the on-state indicative of the location of the subset within the micromirror array. Raman spectra light emitted from the tissue sample is received by at least one light detector as Raman emitted light produced by the beam of light interrogation. The analyzer correlates the assigned location codes with the detector signals representative of the Raman emitted light and produces a spatial map of the Raman emitted light using the correlation.
The disclosed mapping approach is further implemented in a method that provides a tissue sample inoculated with a material containing pHLIPs conjugated with one or more Raman reporters (RR). The interrogation produces Raman emitted light, and the method repeats the DMD scanning with subsets of on-state micromirrors aligned to the beam. The method assigns location codes to the on-state micromirrors, correlates the codes with Raman emitted light signals, and produces the spatial map of Raman emitted light.
Claims Coverage
The partial content identifies two independent claims: a system claim and a method claim. Both center on DMD-based spatial encoding of Raman signals by assigning location codes to on-state micromirror subsets and correlating these codes with detector signals representative of Raman emitted light to produce a spatial Raman map; the method claim additionally specifies pHLIPs conjugated with one or more Raman reporters in an inoculated tissue sample.
DMD subset illumination with location-code assignment for Raman spatial mapping
A system for mapping a tissue sample in which a light source emits a beam of light, a scanner scans a digital mirror device (DMD) having an orthogonal array of micromirrors, the analyzer controls a subset of on-state micromirrors aligned with the beam to direct interrogation to a tissue sample location, assigns one or more location codes to the on-state subset indicative of the subset location within the micromirror array, controls at least one light detector to receive Raman spectra light emitted from the tissue sample and produce signals representative of Raman emitted light, correlates the assigned location codes with the Raman-emitted-light signals, and produces a spatial map of the Raman emitted light using the signals and the correlation.
pHLIPs-conjugated Raman reporters with DMD location-code correlation for spatial Raman mapping
A method of mapping a tissue sample that includes providing a system having a light source, a scanner, a DMD with an orthogonal array of micromirrors, at least one light detector, and an analyzer; providing a tissue sample inoculated with a material containing pHLIPs conjugated with one or more Raman reporters (RR); scanning the DMD with a light beam produced by the light source while using the scanner to move the light beam relative to the DMD; controlling a subset of micromirrors in the on-state aligned with the scanning beam and directing the light beam to interrogate the tissue sample to produce Raman emitted light; assigning one or more location codes to the on-state subset indicative of the subset location within the micromirror array; receiving Raman emitted light using the at least one light detector to produce representative signals; correlating the location codes with the Raman-emitted-light signals; and producing a spatial map of the Raman emitted light using the signals and the correlation.
Across both independent claims, the core inventive structure is the combination of DMD subset control aligned to a scanning beam for interrogating a tissue sample location, assigning location codes to the on-state micromirror subset, and correlating those location codes with detector signals representative of Raman emitted light to produce a spatial map of Raman emitted light; the method claim specifies the tissue sample inoculated with pHLIPs conjugated with Raman reporters.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Wide-field tissue imaging and tumor-margin assessment.
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