High-speed polymerase chain reaction analysis plate
Inventors
Park, Han Oh • Kim, Jong Kab • Lee, Yang Won • PARK, SANG RYOUNG
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Assignees
Member
BioneerBioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Bioneer is a biotechnology company specializing in molecular biology, offering solutions in nucleic acid synthesis, molecular diagnostics, protein production, and laboratory automation. The company's expertise covers oligonucleotide chemistry, gene synthesis, sample preparation, molecular diagnostics, and analytical services such as mass spectrometry and sequencing, serving clinical, research, and industrial sectors globally.
Publication Number
US-12157121-B2
Publication Date
2024-12-03
Expiration Date
Abstract
The present invention relates to the structure of an analysis plate applied to a high-speed polymerase chain reaction (PCR), and to a PCR analysis plate used for implementing an analysis of a real-time PCR, a real-time nested PCR and a post-PCR lateral flow hybridization reaction. The present invention is provided with: a check valve for enabling the maintaining of positive pressure when an elastic film expands into a convex form by having a solution pushed therein by the positive pressure; a lateral flow analysis module for analyzing a post-PCR follow-up PCR or lateral flow; and a shut-off valve enabling the controlling of the movement of the solution after each reaction ends. A high-speed PCR analysis plate may be provided whereby, by pressing, by means of a temperature-controllable heating block, the elastic film, which is in a convex form by the solution, of a PCR unit, a PCR solution may undergo rapid temperature circulation with minimum heat resistance, and a PCR dried material and a nucleic acid solution may be homogenized and mixed.
Core Innovation
The invention relates to a real-time polymerase chain reaction (PCR) analysis plate device. The device includes a base substrate with a reaction unit formed as a closed structure using an elastic sealing film fused into the base substrate in a shape of a closed line, including a first inlet, and a first shut-off valve between the reaction unit and a first flow channel unit connected to the first inlet.
The first shut-off valve is opened by injection of a target nucleic acid solution and then closed by a piston after the injection. Upon injection, the elastic sealing film disposed on the upper portion of the reaction unit is stretched to form an upwardly convex shaped portion due to positive pressure.
The upwardly convex portion of the elastic sealing film is subsequently pressed by a first heating block and a second heating block sequentially. The sequential pressing compresses the upwardly convex portion and enables heat transfer via the compressed convex shaped portion of the elastic sealing film, and the heating by the first heating block and the second heating block heats the nucleic acid solution to enable a PCR reaction.
Claims Coverage
The independent claim is clm-00001, which covers a real-time PCR analysis plate device with injection-actuated valve control, an elastic sealing film formed into an upwardly convex portion under positive pressure, and sequential heating/pressing by first and second movable heating blocks to enable PCR. Additional inventive features are specified in dependent claims that refine structural formats, include lateral flow integration, define label types, and set quantitative constraints for the elastic sealing film geometry or positional parameters.
Elastic sealing film fused into a closed line with an inlet
A reaction unit is formed as a closed structure on the base substrate with an elastic sealing film fused into the base substrate in a shape of a closed line including the first inlet.
Injection opened shut-off valve and piston-closed operation
A first shut-off valve provided between the reaction unit and a first flow channel unit connected to the first inlet is configured to be opened by injection of a target nucleic acid solution and configured to be closed by a piston after the injection.
Upwardly convex elastic film portion formed by positive pressure
By the injection of the target nucleic acid solution, the elastic sealing film disposed on the upper portion of the reaction unit is configured to be stretched to form an upwardly convex shaped portion due to positive pressure.
Sequential heating and pressing using movable first and second heating blocks
The upwardly convex portion of the elastic sealing film of the reaction unit is configured to be subsequently pressed by the first heating block and the second heating block sequentially, thereby compressing the upwardly convex portion and heating the nucleic acid solution by heat transfer via a compressed convex shaped portion of the elastic sealing film to enable a PCR reaction.
Closed-line elastic sealing film projection height constraint
A real-time PCR analysis plate device where the fused projection height of the closed line formed by an elastic sealing film fused into the base substrate is 2 mm or less.
Closed-line width constraint
A real-time PCR analysis plate device where the closed line formed by an elastic sealing film fused to a base substrate has a width of 0.5 mm to 2 mm.
Lateral flow analysis unit coupled via a second flow channel and controlled by a shut-off valve
An outlet leading from the reaction unit to a lateral flow analysis unit via a second flow channel, where the lateral flow unit contains a fixed nucleic acid probe and an elastic sealing film formed into a closed-line shape with a second inlet, and where a first shut-off valve intermittently controls target nucleic acid solution flow through the second flow channel to the lateral flow inlet.
Labeled nested primer using fluorescent, chemiluminescent, or gold nanoparticle label materials
The device uses a labeled nested primer labeled with a fluorescent material, a chemiluminescent material, or gold nanoparticles.
Relative temperature relationship between heating blocks
The device includes first and second heating blocks where the first heating block operates at a higher temperate than the second heating block.
Across the independent and dependent claims, the core coverage centers on a closed reaction unit with an elastic sealing film fused into a closed line, injection-actuated shut-off valve control closed by a piston, formation of an upwardly convex film portion by positive pressure, and sequential pressing/heating by first and second movable heating blocks to enable PCR. Dependent claims further narrow the elastic film geometry and add a lateral flow analysis unit and specified label types, as well as define a relative temperature relationship between heating blocks.
Stated Advantages
Enables a PCR reaction by heating the nucleic acid solution through heat transfer via a compressed convex shaped portion of the elastic sealing film.
Documented Applications
Real-time PCR analysis using the described analysis plate device.
Real-time nested PCR using a second PCR unit connected through a flow channel.
Multiplex nested PCR with parallel secondary PCR units.
Post-PCR lateral flow analysis by integrating a lateral flow analysis module with fixed nucleic acid probes and qualitative detection using labels.
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