System and method for the production of biomolecules such as viral vaccines
Inventors
Castillo, José • Mairesse, Bastien • De Viron, Laetitia • Drugmand, Jean-Christophe
Assignees
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Publication Number
US-12152231-B2
Publication Date
2024-11-26
Expiration Date
Abstract
The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.
Core Innovation
A fixed bed bioreactor includes a chamber for receiving a liquid and is adapted for producing target biomolecules. A growth medium tank provides a growth medium to the bioreactor, and an intermediate vessel receives concentrated target biomolecules for harvest.
A concentrator separates the target biomolecule for concentration in the intermediate vessel for harvest. The system includes conduits for transfer between the chamber of the bioreactor and the intermediate vessel, transfer between the intermediate vessel and the concentrator, recycling liquid exiting the bioreactor back to the growth medium tank for redelivery into the bioreactor, recirculation of retentate to an input of the intermediate vessel, and discarding permeate to a waste container.
For purification, the upstream process compartment comprises a fixed-bed perfusion bioreactor with a spiral structure for allowing growth of cells and promoting fluid distribution and turbulence. Additional compartments include a clarification compartment, a chromatography compartment, and a viral inactivation compartment, and the compartments are located in one or more cabinets, isolators, or containment enclosures.
Claims Coverage
The document contains four independent claims. Across these claims, the inventive features include a fixed-bed bioreactor coupled to an intermediate vessel via a concentrator, conduit-based recycling and retentate recirculation, permeate discard to a waste container, and downstream clarification, chromatography, and viral inactivation housed in cabinets, isolators, or containment enclosures.
Fixed-bed bioreactor with intermediate vessel and concentrator harvest
A fixed bed bioreactor including a chamber for receiving a liquid, a growth medium tank, an intermediate vessel, and a concentrator adapted to separate the target biomolecule for concentration in the intermediate vessel for harvest.
Conduit network with bioreactor redelivery and retentate recirculation
A plurality of conduits including transfer between the bioreactor chamber and the intermediate vessel, transfer between the intermediate vessel and the concentrator, recycling liquid exiting the bioreactor back to the growth medium tank for redelivery into the bioreactor, and retentate recirculation to an input of the intermediate vessel.
Permeate discard conduit to waste container
An outlet conduit from the concentrator to the waste container to discard a permeate from the concentrator.
Spiral fixed-bed perfusion bioreactor for fluid distribution and turbulence
An upstream process compartment comprising a fixed-bed perfusion bioreactor including a chamber for receiving a liquid and producing target biomolecules and provided with a spiral structure for allowing growth of cells and promoting fluid distribution and turbulence.
Upstream purification flow with clarification, chromatography, and viral inactivation
A clarification compartment adapted to receive the concentrated target biomolecules, a chromatography compartment, and a viral inactivation compartment.
Compartment containment in cabinets, isolators, or containment enclosures
The compartments are located in one or more cabinets, isolators, or containment enclosures.
Dedicated harvest conduit from intermediate vessel
A harvest conduit adapted to harvest concentrated target biomolecules from the intermediate vessel.
The independent claims collectively cover systems that produce or purify concentrated target biomolecules using a fixed-bed bioreactor with concentrator-based harvest through an intermediate vessel, supported by conduit-based transfer, recycling, and retentate recirculation, with permeate discarded to a waste container. Purification embodiments additionally define clarification, chromatography, and viral inactivation compartments and require housing of compartments in cabinets, isolators, or containment enclosures.
Stated Advantages
Documented Applications
No documented applications found
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