Chelator-coated field effect transistor and devices and methods using same
Inventors
Assignees
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Publication Number
US-12111313-B2
Publication Date
2024-10-08
Expiration Date
Abstract
A field effect transistor comprising a chelator or derivatized chelator, a device for detecting a marker in a sample comprising said sensor, a method for detecting metal ions in a sample comprising said sensor or device and a sensor and method for detecting a marker in a sample.
Core Innovation
The invention provides a method for the detection of a target marker in a solution including charged ions and unrelated ions by combining a liposome-based metal-ion label with a field-effect transistor sensor. The method includes providing a liposome in solution containing metal ions, where the liposome includes a detection antibody configured to bind a target marker. The target marker is selectively bound so that the bound liposome is disposed on the source-drain channel of the field-effect transistor.
A functionalized surface on the FET source-drain channel includes both a capture antibody to selectively bind to the target marker and a metal ion chelator or metal ion derivatized chelator. After the target marker is bound and disposed on the FET source-drain channel, the method releases the metal ions from the liposome and selectively binds the released metal ions with the metal ion chelator or metal ion derivatized chelator disposed on the functionalized surface.
The selective binding at the FET surface causes a change in current in the field-effect transistor, and the change in current is indicative of detection of the target marker. The approach addresses detection in ion-containing solutions by using chelation at a chelator or derivatized chelator functionalized surface coupled to a current readout from the FET, linked to a liposome carrying a metal-ion label, without interference from screening oppositely charged ions and unrelated ions in the solution.
Claims Coverage
The document includes one independent claim describing the overall detection method, with dependent claims refining inventive features such as the selection of chelators, metal ions, transistor substrate material, release mechanism, and target marker categories. The independent claim includes multiple inventive features that together enable current change detection without interference from charged ions and unrelated ions.
Liposome metal-ion label with detection antibody
providing a liposome in solution containing metal ions, the liposome including a detection antibody configured to bind a target marker.
FET source-drain functionalized with capture antibody and metal-ion chelator
providing a field-effect transistor with a source-drain channel comprising a surface functionalized with both a capture antibody to selectively bind to the target marker and a metal ion chelator or metal ion derivatized chelator.
Target marker bridging and placement on FET source-drain channel
selectively binding the target marker to the detection antibody included with the liposome; selectively binding the target marker to the capture antibody to dispose the target marker and bound liposome to the source-drain channel of the field-effect transistor.
Chelator-mediated metal-ion release and current change readout
releasing the metal ions from the liposome; selectively binding the metal ion chelator or metal ion derivatized chelator disposed on the functionalized surface of the source-drain channel with the metal ions released from the liposome; and causing a change in current in the field-effect transistor as a result of the selective binding of the metal ion chelator or metal ion derivatized chelator with the metal ions released from the liposome.
Screening-resistant detection in charged-ion solutions
causing a change in current in the field-effect transistor as a result of the selective binding of the metal ion chelator or metal ion derivatized chelator with the metal ions released from the liposome without interference of the detection of the metal ions by screening oppositely charged ions and unrelated ions in the solution, wherein the change in current is indicative of detection of the target marker.
Overall claim coverage centers on transferring a liposome-associated metal-ion detection label onto a chelator-and-capture-antibody functionalized FET surface via a target marker, releasing and chelating the released metal ions at the source-drain channel, and using the resulting FET current change as an indicator of detection while avoiding interference from screening ions.
Stated Advantages
Detection of metal ions and the target marker in an ion-containing solution without interference of metal-ion detection by screening oppositely charged ions and unrelated ions.
The change in current in the field-effect transistor is indicative of detection of the target marker.
Documented Applications
Detection of a target marker in a solution including charged ions and unrelated ions, with target marker categories including biomarkers, environmental markers, allergens, or microorganisms.
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