Methods of processing a biofluid sample
Inventors
Blume, John E. • Manning, William C. • Troiano, Gregory • Siddiqui, Asim • Ma, Philip • Farokhzad, Omid C.
Assignees
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Publication Number
US-12087405-B2
Publication Date
2024-09-10
Expiration Date
Abstract
Disclosed herein are methods and compositions for processing biofluid samples. Some such methods may include obtaining a biofluid sample from a subject having a disease state such as lung cancer. The biofluid sample may be contacted with a nanoparticles to adsorb proteins. The proteins may then be ionized or contacted with a detection reagent. Also disclosed herein are compositions comprising proteins coupled to a nanoparticle upon contact of the nanoparticle with a biofluid sample from a subject having a disease.
Core Innovation
A method contacts a biofluid sample from a subject suspected of having non-small cell lung cancer (NSCLC) or a comorbidity of NSCLC with particles to form biomolecule coronas comprising proteins of the biofluid adsorbed to the particles. The proteins comprise one or more of HTRA1, PXDN, ANTR2, CSPG2, ANTR1, NOTUM, CILP1, NCAM1, RLA2, S10A8, S10A9, TBA1A, and CAN2.
The method obtains a data set by identifying and measuring the proteins of the biomolecule coronas. The data set is input into a classifier that distinguishes between NSCLC and the comorbidity of NSCLC, and the data set is identified as indicative of NSCLC or the comorbidity of NSCLC through the classifier.
The classification result is used in connection with administering a cancer treatment to the subject. The cancer treatment comprises an anti-cancer agent, radiation, or cancer resection surgery.
Claims Coverage
The document includes one independent claim covering three inventive features: corona formation from subject biofluid proteins on particles, measurement and classification of corona proteins to distinguish NSCLC from an NSCLC comorbidity, and administration of cancer treatment based on the classification.
Biomolecule coronas from biofluid proteins on particles
Contacting a biofluid sample from a subject suspected of having NSCLC or a comorbidity of NSCLC with particles to form biomolecule coronas comprising proteins of the biofluid adsorbed to the particles, wherein the proteins comprise one or more of HTRA1, PXDN, ANTR2, CSPG2, ANTR1, NOTUM, CILP1, NCAM1, RLA2, S10A8, S10A9, TBA1A, and CAN2.
Protein corona dataset identification and measurement
Obtaining a data set by identifying and measuring the proteins of the biomolecule coronas.
Classifier distinguishing NSCLC from NSCLC comorbidity
Inputting the data set into a classifier that distinguishes between NSCLC and the comorbidity of NSCLC.
Classifier-indicative diagnosis used to administer cancer treatment
Identifying the data set as indicative of NSCLC or the comorbidity of NSCLC through the classifier and administering a cancer treatment to the subject, the cancer treatment comprising an anti-cancer agent, radiation, or cancer resection surgery.
The core coverage is the corona-based measurement of specific protein sets from a biofluid sample, producing a dataset that a classifier uses to distinguish NSCLC from an NSCLC comorbidity, and using that classification to support administering anti-cancer agent, radiation, or resection surgery.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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