Device to be used for capturing extracellular vesicles, and preservation method and transport method for extracellular vesicles
Inventors
Yasui, Takao • Baba, Yoshinobu • Koga, Hirotaka
Assignees
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Publication Number
US-12038360-B2
Publication Date
2024-07-16
Expiration Date
Abstract
The present invention provides a novel miRNA extraction method and a method for analyzing miRNA extracted by using said miRNA extraction method. According to the present invention, provided is, for example, a method for extracting miRNA from extracellular vesicles in a sample solution, by using a device capable of capturing extracellular vesicles, the miRNA extraction method comprising: an extracellular vesicle capturing step for capturing extracellular vesicles in a sample solution onto a device by bringing the sample solution and the device in contact with each other; and a miRNA extraction step for homogenizing the extracellular vesicles by bringing the device having captured the extracellular vesicles in contact with a homogenization liquid for extracellular vesicles to extract miRNA from the extracellular vesicle into the homogenization liquid.
Core Innovation
The invention relates to an extracellular vesicle capture device including a nanostructure body made using cellulose nanofibers, such as cellulose nanofiber/nanocellulose nanoporous films and related nanostructures. The nanostructure body includes nanopores or nanowires and is used to capture extracellular vesicles from a sample solution by bringing the device and the sample solution into contact.
The invention further provides methods in which extracellular vesicle capture is followed by drying that removes liquid of the sample solution which impregnates the device, and then preserving that includes preserving the dried device having captured the extracellular vesicles. The disclosed approach is directed to capturing extracellular vesicles without extracellular vesicle fractionation and supporting miRNA extraction from extracellular vesicles.
In addition, the invention discloses preserving and transfer by drying on the nanoporous cellulose nanofibers device and using room-temperature stability for subsequent use, including transfer by mailing the dried device at room temperature. The partial content indicates improved stability with nanopores compared with devices without nanopores, and includes miRNA profiling results using small sample volumes from saliva and urine.
Claims Coverage
The partial content includes three independent claims covering capture and drying on a cellulose-nanofiber nanostructure device, with preservation, transfer, or miRNA extraction as the downstream feature.
Cellulose-nanofiber nanostructure EV capture, drying, and preservation
A method of preserving extracellular vesicles comprising capturing an extracellular vesicle in the sample solution in a device having a nanostructure body made using cellulose nanofibers, drying by removing liquid impregnating the device, and preserving the dried device having captured the extracellular vesicle.
Cellulose-nanofiber nanostructure EV capture, drying, and transfer
A method of transferring an extracellular vesicle comprising capturing an extracellular vesicle in the sample solution in a device having a nanostructure body made using cellulose nanofibers, drying by removing liquid impregnating the device, and transferring the dried device having captured the extracellular vesicle.
Cellulose-nanofiber nanostructure EV capture, drying, and miRNA extraction
A method of extracting miRNA from extracellular vesicles comprising capturing an extracellular vesicle in a device having a nanostructure body made using cellulose nanofibers, drying by removing liquid impregnating the device, preserving the dried device having captured the extracellular vesicle, and extracting miRNA from the captured extracellular vesicle.
Across the independent claims, the inventive coverage centers on using a device with a nanostructure body made using cellulose nanofibers for extracellular vesicle capture, followed by drying and then either preserving the dried capture device, transferring the dried capture device, or preserving and then extracting miRNA from extracellular vesicles captured on the dried device.
Stated Advantages
Improved stability with nanopores compared with devices without nanopores.
Room-temperature stability for dried extracellular vesicle capture devices.
Direct miRNA extraction from extracellular vesicles without extracellular vesicle fractionation.
Documented Applications
miRNA profiling from extracellular vesicles captured from saliva and urine using small sample volumes.
Extracellular vesicle preservation by drying on a nanoporous cellulose/nanofiber device with room-temperature stability.
Extracellular vesicle transfer by mailing the dried device at room temperature.
Extracellular vesicle and miRNA workflows using a cellulose nanofiber nanostructure capture device following drying and preserving before downstream use.
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