Amplification module with gas moving passage and extract moving passage

Inventors

Cho, Young-Shik • Park, Hae-Joon • Lee, Sunyoung • LIM, Kwanhun • Kim, Dong-hun • KIM, In-Ae • PARK, Hyo-Lim

Assignees

SD Biosensor Inc

Abstract

Provided is an amplification module with a gas moving passage and an extract moving passage, more particularly an amplification module in which, when an extract is input from a genome extraction device in which the amplification module is installed, a quantitative amount of the extract is input to each accommodating portion so that the accuracy of detection can be increased.

Core Innovation

The invention relates to a genome extraction device connected to an amplification module. The amplification module accommodates an extract extracted from the genome extraction device, and the extract is introduced into one or more accommodating portions through passage structures that provide quantitatively controlled extract introduction.

The amplification module includes a body with at least two injection ports formed on a first side that communicate with discharge holes of the genome extraction device. The body further includes a gas moving passage and an extract moving passage, where the gas moving passage is configured to connect injection ports to accommodating portions and the extract moving passage is configured to connect corresponding injection ports to the accommodating portions.

The accommodating portion is provided in a plurality, and the gas moving passage extends from a first injection port to correspond to the plurality of accommodating portions in a one-to-one correspondence, while the extract moving passage extends from a second injection port to correspond to the plurality of accommodating portions in a one-to-one correspondence. To reduce bubbles/air, prevent mixing, and prevent cross-contamination between neighboring accommodating portions, the body and passages include structural shape features such as rounded edges, curved passages, and tapered accommodating portions.

Different accommodating portions can accommodate primers/probes and/or amplification materials for amplifying different genomes, enabling simultaneous detection. The background problems addressed include leakage, cross-contamination, bubble generation, and moisture sensitivity issues associated with beads, and the solution-level approach includes sealing members, a safety clip, and flow-path design for moisture control and vacuum/air management.

Claims Coverage

The partial document identifies one independent claim (clm-00001) directed to an amplification module connected to a genome extraction device with separate gas and extract flow passages that route in a one-to-one correspondence to multiple accommodating portions. Dependent claim refinements add positional separation (upper/lower portions), passage curvature with rounded edges, tapering shapes for accommodating portions, and configuration that supports storing amplification materials for different genome targets.

Overall, the claim set coverage in the partial content focuses on an amplification module architecture that routes gas and extract through separate passage structures to multiple accommodating portions with one-to-one correspondence, while dependent refinements add upper/lower positional separation, curved passages with rounded edges, tapering accommodating portions, and accommodating portions configured to store amplification materials for amplifying extracted genomes.

Stated Advantages

Documented Applications