Ion selective sensor
Inventors
ABEYRATHNE, Chathurika Darshani • LIANG, You • Skafidas, Efstratios
Assignees
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Publication Number
US-12019045-B2
Publication Date
2024-06-25
Expiration Date
Abstract
An ion sensor includes a substrate, at least one ion selective electrode deposited on the substrate, and a reference electrode deposited on the substrate. The sensor may further include an insulating layer placed over the ion selective electrode and the reference electrode and having openings for the ion selective electrode and the reference electrode, a microfluidic layer placed over at least part of the insulating layer, and a cover layer placed over the microfluidic layer. The reference electrode includes reference electrode material deposited on the substrate and a combination of a polymer and a chloride-containing salt deposited on the reference electrode material.
Core Innovation
The invention relates to an ion sensor comprising a substrate comprising a non-conductive material, at least one ion selective electrode comprising an ion selective electrode material deposited on the substrate, and a reference electrode comprising reference electrode material deposited on the substrate. The reference electrode further comprises a combination of a chloride-containing salt and a polymer deposited on the reference electrode material, and the sensor includes an insulating layer placed over the at least one ion selective electrode and the reference electrode, where the insulating layer comprises at least one opening for each of the at least one ion selective electrode and the reference electrode.
Over at least part of the ion selective electrode material, an ion-selective membrane is disposed. The ion-selective membrane comprises an ionophore protein cocktail configured to detect a specific antibody, and a microfluidic layer is placed over at least part of the insulating layer, with a cover layer placed over the microfluidic layer.
The described sensor architecture includes a layered, planar solid-state configuration with deposited planar electrodes and membrane-based functionality, including optional structures such as an insulating layer with electrode openings, a microfluidic layer, and a cover layer optionally including an exit pore/inlet lip, and optionally a filter material layer positioned relative to the insulating layer, microfluidic layer, and cover layer. The document also describes reference-electrode stability and ion selectivity results showing that interfering Na+ and Ca2+ do not shift signal, with high correlation with a standard ion meter for K+ and Na+ measurements in sweat and saliva.
Claims Coverage
The partial content provides two independent claims: an ion sensor apparatus and a method for making that ion sensor. Across these independent claims, the coverage centers on a planar, stacked electrode/membrane/microfluidic structure with an insulating layer having openings, and a solid-state reference electrode formed from deposited reference electrode material plus a chloride-containing salt and polymer, with an ion-selective membrane comprising an ionophore protein cocktail for detecting a specific antibody.
Ion sensor with deposited planar electrodes and solid-state reference electrode chemistry
An ion sensor comprising a substrate comprising a non-conductive material; at least one ion selective electrode comprising an ion selective electrode material deposited on the substrate; a reference electrode comprising a reference electrode material deposited on the substrate; and a combination of a chloride-containing salt and a polymer deposited on the reference electrode material, plus an insulating layer placed over the at least one ion selective electrode and the reference electrode with at least one opening for each of the at least one ion selective electrode and the reference electrode.
Ion-selective membrane with ionophore protein cocktail for antibody detection in an integrated microfluidic stack
An ion-selective membrane disposed over at least a part of the ion selective electrode material, wherein the ion-selective membrane comprises an ionophore protein cocktail configured to detect a specific antibody; a microfluidic layer placed over at least part of the insulating layer; and a cover layer placed over the microfluidic layer.
Method for making an ion sensor with deposited electrodes, chloride salt/polymer reference electrode, and layered microfluidic/cover stack
A method for making an ion sensor comprising providing a substrate comprising a non-conductive material; forming at least one ion selective electrode on the substrate by depositing at least one ion selective electrode material on the substrate and positioning an ion-selective membrane over part of the at least one ion selective electrode material; forming a reference electrode on the substrate by depositing a reference electrode material on the substrate and depositing a combination of a polymer and a chloride-containing salt on the reference electrode material; placing an insulating layer over the at least one ion selective electrode and the reference electrode, the insulating layer comprising at least one opening for each of the at least one ion selective electrode and the reference electrode; placing a microfluidic layer over at least part of the insulating layer; and placing a cover layer over the microfluidic layer.
Ion-selective membrane in the manufacturing method configured to detect a specific antibody
Wherein the ion-selective membrane comprises an ionophore protein cocktail configured to detect a specific antibody.
In the independent claim coverage, the core inventive combination is an ion sensor built from deposited planar ion selective electrode(s) and a solid-state reference electrode formed from deposited reference electrode material plus a chloride-containing salt and polymer, integrated with an insulating layer having openings, and a layered microfluidic and cover stack. The ion-selective membrane is configured to detect a specific antibody via an ionophore protein cocktail.
Stated Advantages
Reference-electrode stability.
Ion selectivity, where interfering Na+ and Ca2+ do not shift signal.
High correlation with a standard ion meter (R-square > 0.9) for K+ and Na+ measurements.
Documented Applications
K+ and Na+ measurements in sweat.
K+ and Na+ measurements in saliva.
pH sensor use case including measurement using an ion sensor architecture [procedural detail omitted for safety].
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