Pseudomonas protegens and products thereof to control bacterial panicle blight of rice
Inventors
Rojas, Clemencia M. • Rojas, J. Alejandro • Lay, Jr., Jackson O.
Assignees
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Publication Number
US-11999963-B2
Publication Date
2024-06-04
Expiration Date
Abstract
The present invention provides biosynthetic products that may be useful for controlling bacterial panicle blight of rice. These products are encoded by four biosynthetic operons found within the genome of a Pseudomonas protegens strain designated as PBL3 and deposited as ATCC #, which has been shown to inhibit the growth of Burkholderia glumae on rice plants. Also provided are vectors encoding these biosynthetic products, and methods of using the biosynthetic products to inhibit the growth of microorganisms.
Core Innovation
The disclosure addresses bacterial panicle blight (BPB) of rice caused by Burkholderia glumae and provides a biocontrol approach based on Pseudomonas protegens strain designated as PBL3 and deposited as NRRL Accession No. B-68083. The invention identifies biosynthetic gene clusters in PBL3 for secondary metabolites orfamide A/C, pyoluteorin, and pyrrolnitrin (SEQ ID NO:1-3), and the biosynthetic products are produced by PBL3 and are used to inhibit the growth of Burkholderia glumae.
Experimental results described in the disclosure indicate that PBL3 antagonizes B. glumae in vitro and reduces disease symptoms in rice. The activity is associated with the cell-free secreted fraction and is retained after boiling and protease treatment, and sub-fractionation indicates multiple bioactive molecules across molecular-weight ranges.
Treated secretions reduce B. glumae growth and impair pathogenicity at early stages while reducing lesions and protecting seed germination/growth.
Claims Coverage
The disclosure contains one independent claim directed to inhibiting the growth of Burkholderia glumae by contacting with a biosynthetic product produced by Pseudomonas protegens strain PBL3 (NRRL Accession No. B-68083). The independent claim includes three inventive feature elements.
Contacting Burkholderia glumae with an effective amount of a biosynthetic product to inhibit growth
A method of inhibiting the growth of Burkholderia glumae by contacting the Burkholderia glumae with an effective amount of a biosynthetic product, thereby inhibiting its growth.
Using Pseudomonas protegens strain PBL3 deposited as NRRL Accession No. B-68083 as the producer
The biosynthetic product is produced by a Pseudomonas protegens strain designated as PBL3 and deposited as NRRL Accession No. B-68083.
Selecting the biosynthetic product as orfamide A/C, pyoluteorin, or pyrrolnitrin
The biosynthetic product is selected from orfamide A/C, pyoluteorin, and pyrrolnitrin.
Overall, the claim coverage is centered on inhibiting Burkholderia glumae growth by contacting it with an effective amount of a biosynthetic product produced by Pseudomonas protegens PBL3 (NRRL Accession No. B-68083), where the product is selected from orfamide A/C, pyoluteorin, and pyrrolnitrin.
Stated Advantages
Inhibits the growth of Burkholderia glumae.
Reduces disease symptoms in rice.
Activity is associated with the cell-free secreted fraction and is retained after boiling and protease treatment.
Reduces lesions and protects seed germination/growth.
Documented Applications
Biocontrol of bacterial panicle blight of rice caused by Burkholderia glumae using Pseudomonas protegens strain PBL3 biosynthetic products (orfamide A/C, pyoluteorin, pyrrolnitrin).
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