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Publication Number
US-11946068-B2
Publication Date
2024-04-02
Expiration Date
Abstract
A system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold includes a first chamber for accommodating the scaffold, a TAI storage device for storing TAIs, at least one second chamber for storing TAI media, and a gas inlet for receiving gas from a compressed source. A flow circuit is coupled to the first chamber, the TAI storage device, the second chamber and the gas inlet for delivering the TAIs, the TAI media and the gas to the scaffold. A pump pumps at least one of the TAIs, the TAI media and the gas in the flow circuit. The system also includes a processor that regulates the delivery of the TAIs, the TAI media and the gas to the scaffold via a plurality of valves.
Core Innovation
The disclosed subject matter describes an automated system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold and for regulating subsequent culturing of the porous scaffold. The system includes a first chamber for accommodating the porous scaffold, a second chamber for storing TAI media, and a TAI storage device for storing TAIs, together with a gas inlet configured to receive gas from a compressed source. A flow circuit couples the first chamber, the TAI storage device, the second chamber, and the gas inlet so that the flow circuit delivers the TAIs, the TAI media, and the gas to the porous scaffold.
A pump is provided for pumping at least one of the TAIs, the TAI media, and the gas in the flow circuit, and a plurality of valves regulates delivery of the TAIs, the TAI media, and the gas to the porous scaffold. A processor is programmed to regulate seeding and culturing by switching one or more valves between open and closed positions. The valve set includes a media valve, a gas valve, a TAI valve, a first chamber valve, and a waste valve, enabling coordinated control of flows to and from the porous scaffold.
The processor performs a pre-wetting process for pre-wetting the porous scaffold with the TAI media using a multi-step valve switching routine. The pre-wetting process includes pumping TAI media into the first chamber with the gas valve and the TAI valve in closed position while the media valve, first chamber valve, and waste valve are open, pumping gas into the first chamber with the gas valve and the first chamber valve open while the media valve and the TAI valve are closed, and regulating pulsing pressurized gas bursts through the porous scaffold by repeatedly alternating the gas valve, the first chamber valve, and the waste valve between open and closed positions while the media valve and the TAI valve remain in closed position.
Claims Coverage
The independent claims identified are clm-00001 and clm-00014. Each independent claim is directed to a system or a method for seeding TAIs into a porous scaffold using a flow circuit, a pump, multiple valves, and a processor-controlled pre-wetting process that includes pulsing pressurized gas bursts.
Processor-regulated system delivering TAIs, TAI media, and gas via a flow circuit and plurality of valves
A system for seeding therapeutic active ingredients (TAIs) onto a porous scaffold comprising a first chamber, a TAI storage device, a second chamber for TAI media, a gas inlet receiving gas from a compressed source, a flow circuit coupled to the first chamber, TAI storage device, second chamber, and gas inlet delivering the TAIs, the TAI media and the gas to the porous scaffold, a pump pumping at least one of the TAIs, the TAI media and the gas, and a plurality of valves for regulating delivery of the TAIs, the TAI media and the gas to the porous scaffold; and a processor programmed to regulate seeding and culturing by switching one or more valves between open and closed positions.
Pre-wetting process using sequential media pumping, gas pumping, and pulsing pressurized gas bursts with coordinated valve switching
The processor is programmed to perform a pre-wetting process for pre-wetting the porous scaffold with the TAI media, the pre-wetting process comprising (i) setting the media valve, the first chamber valve, and the waste valve to an open position while the gas valve and the TAI valve are in a closed position to pump the TAI media into the first chamber; (ii) setting the gas valve, the first chamber valve, and the waste valve to an open position while the media valve and the TAI valve are closed to pump the gas into the first chamber; and (iii) regulating pulsing pressurized gas bursts through the porous scaffold by repeatedly alternating each of the gas valve, the first chamber valve, and the waste valve between an open position and a closed position while the media valve and the TAI valve are in a closed position.
Method for seeding and culturing TAIs into a porous scaffold with pump and processor-regulated valve delivery including pre-wetting
A method for seeding and culturing therapeutic active ingredients (TAIs) into a porous scaffold comprising accommodating the porous scaffold in a first chamber; delivering TAIs, TAI media and gas to the porous scaffold via a flow circuit from a TAI storage device, at least one second chamber and a gas inlet; pumping at least one of the TAIs, the TAI media and the gas in the flow circuit by a pump; and regulating a delivery of the TAIs, the TAI media and the gas to the porous scaffold by a processor via a plurality of valves including a media valve, a gas valve, a TAI valve, a first chamber valve, and a waste valve.
Pre-wetting process steps for the method including sequential media pumping, gas pumping, and pulsed pressurized gas bursts
The processor performs a pre-wetting process for pre-wetting the porous scaffold with the TAI media, comprising steps of (i) setting the media valve, the first chamber valve, and the waste valve to an open position while the gas valve and the TAI valve are in a closed position to pump the TAI media into the first chamber; (ii) setting the gas valve, the first chamber valve, and the waste valve to an open position while the media valve and the TAI valve are closed to pump the gas into the first chamber; and (iii) regulating pulsing pressurized gas bursts through the porous scaffold by repeatedly alternating each of the gas valve, the first chamber valve and the waste valve between an open position and a closed position while the media valve and the TAI valve are in a closed position.
Across both independent claims, the inventive coverage centers on a processor-controlled delivery architecture using a flow circuit, a pump, and multiple valves to coordinate delivery of TAIs, TAI media, and gas to a porous scaffold, together with a processor-performed pre-wetting process that includes sequential media pumping, gas pumping, and pulsing pressurized gas bursts via coordinated valve open/closed switching.
Stated Advantages
Documented Applications
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