Synthetic human cell mimic hydrogel particle for cytometric or coulter device
Inventors
Kim, Jeffrey • LIU, OLIVER • Agresti, Jeremy • Nguyen, Anh Tuan
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11927519-B2
Publication Date
2024-03-12
Expiration Date
Abstract
Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.
Core Innovation
The invention provides synthetic human cell mimic hydrogel particles comprising a hydrogel particle with a polymerized monomer. The particles possess an optical property, or a volumetric or capacitance property, substantially similar to a corresponding property of a natural biological cell counterpart. These particles serve as synthetic calibrants and assay references to address problems associated with reliance on variable purified-cell standards.
The particles are tunable to mimic target-cell optical properties, including forward scatter, side scatter, and fluorescence, as well as volumetric or capacitance properties. Tuning is achieved through polymer composition, particle size, embedded materials including silica nanoparticles, and surface functionalization with controlled amounts of cell-surface markers. The resulting compositions are formulated for use in cytometric devices and Coulter counters to reproduce cell populations.
Claims Coverage
The independent claims cover two inventive features directed to synthetic human cell mimic hydrogel particles.
Optical-property-matched hydrogel particle
Claim 1 recites a synthetic human cell mimic hydrogel particle comprising a hydrogel particle comprising a polymerized monomer, wherein the particle comprises an optical property substantially similar to a corresponding optical property of its natural biological cell counterpart.
Volumetric- or capacitance-property-matched hydrogel particle
Claim 19 recites a synthetic human cell mimic hydrogel particle comprising a hydrogel particle comprising a polymerized monomer, wherein the particle comprises a volumetric or capacitance property substantially similar to a corresponding property of its natural biological cell counterpart.
The two independent claims cover synthetic human cell mimic hydrogel particles configured to match natural biological cell counterparts in optical properties or in volumetric or capacitance properties.
Stated Advantages
The synthetic calibrants reduce reliance on variable purified-cell standards.
Documented Applications
The particles are used for flow-cytometry calibration, marker-detection sensitivity standards, and flow-cytometry scatter analysis distinguishing lymphocyte, monocyte, and granulocyte populations.
The compositions comprising the particle are formulated for use as synthetic calibrants and assay references in cytometric devices and Coulter counter devices.
Interested in licensing this patent?