All-electronic high-throughput analyte detection system
Inventors
QUEVY, Emmanuel Philippe • Gupta, Chaitanya • HUI, Jeremy
Assignees
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Publication Number
US-11921077-B2
Publication Date
2024-03-05
Expiration Date
Abstract
An all-electronic high-throughput detection system can perform multiple detections of one or more analyte in parallel. The detection system is modular, and can be easily integrated with existing microtiter plate technologies, automated test equipments and lab workflows (e.g., sample handling/distribution systems). The detection system includes multiple sensing modules that can perform separate analyte detection. A sensing module includes a platform configured to couple to a sample well. The sensing module also includes a sensor coupled to the platform. The sensing module further includes a first electrode coupled to the platform. The first electrode is configured to electrically connect with the sensor via a feedback circuit. The feedback circuit is configured to provide a feedback signal via the first electrode to a sample received in the sample well, the feedback signal based on a potential of the received sample detected via a second electrode.
Core Innovation
The invention provides an all-electronic, modular high-throughput analyte detection system using an array of sensing modules. Each sensing module is compatible with a sample well or platform such as a microtiter plate and vials, and it is configured for automated workflows.
In the system, a potential associated with a sample is detected using a first electrode. The feedback circuit electrically coupled to the first electrode generates a feedback signal, and the feedback signal is delivered to the sample via a second electrode, configured to provide excitation control of redox species in the sample at a third electrode, forming a three-electrode feedback topology.
The system is also configured to detect analyte-indicative current from the working electrode as part of the feedback operation. It supports scalability using readout channels and time-multiplexed operation via a switching matrix, and the platform may include electromagnetic shields and optional vial/cap electrode structures.
Claims Coverage
The independent claim recites three main inventive aspects: potential detection, feedback-signal generation, and feedback delivery for excitation control using first, second, and third electrodes on a platform. Dependent claim coverage further specifies analyte-indicative current detection, platform electromagnetic shielding, removable cap-based electrode coupling, electrode mounting location, and defined electrode geometry.
Potential detection via first electrode in sample well
Detecting a potential associated with a sample received in a sample well by a first electrode.
Feedback signal generation by feedback circuit coupled to first electrode
Generating a feedback signal by a feedback circuit electrically coupled to the first electrode.
Feedback delivery via second electrode for excitation control at third electrode
Providing the feedback signal to the sample via a second electrode, the feedback signal configured to provide excitation control of redox species in the sample at a third electrode.
Three electrodes coupled to platform configured to receive the sample well
The first, the second and the third electrodes are coupled to a platform configured to receive the sample well.
Analyte-indicative current detection using third electrode
The feedback circuit is configured to detect a current from the sample using a third electrode, wherein the detected current indicates an analyte in the sample.
Electromagnetic shield on platform attenuating external electromagnetic radiation
The platform has an electromagnetic shield configured to protect the sensor by attenuating external electromagnetic radiation.
Removably coupled first electrode on a cap
The first electrode is positioned on a cap configured to removably couple to the sample well.
First electrode mounted on a wall of the sample well
The first electrode is mounted on a wall of the sample well.
Second electrode surface across sample well substantially parallel to platform
The second end includes a surface extending across the sample well, with that surface and the platform substantially parallel to one another.
The claim coverage centers on detecting a sample potential and using a feedback circuit to generate and deliver a feedback signal through additional electrodes to provide excitation control of redox species at a third electrode. The dependent claims extend this to analyte-indicative current detection, electromagnetic shielding, cap-based removable electrode coupling, electrode mounting location, and electrode geometry.
Stated Advantages
Noise/thermal-noise suppression using a three-electrode feedback topology.
Room-temperature operation using lock-in detection.
Scalability via readout channels.
Time-multiplexed operation via a switching matrix.
Protection by electromagnetic shielding through attenuation of external electromagnetic radiation.
Documented Applications
Biosensing example using whole blood with a potassium ferri-/ferrocyanide redox couple.
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