Platforms and systems for automated cell culture
Inventors
Wagner, Matthias • Aivio, Suvi • AMENDUNI, Mariangela • PILSMAKER, Catherine • Pereira, Arnaldo • ZUTSHI, Ananya • TOURE, Anthia • Nagle, Steven • Whiting, Ozge • Harb, George • Sullivan, Matthew • BERLIN-UDI, Maya • Morgan, Stefanie • SEAY, Nick • Lee, Sang • LURO, Scott
Assignees
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Publication Number
US-11913029-B2
Publication Date
2024-02-27
Expiration Date
Abstract
Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.
Core Innovation
The invention relates to a modular bioprocessing system that performs cell culture processes using one or more cell culture process modules. Each module receives one or more cell culture containers, and each container includes at least one semi-transparent surface permanently attached thereto. The semi-transparent surface enables light-based cell imaging when illuminated with light within a first wavelength range and enables light-based cell manipulation when illuminated with light within a second wavelength range.
The light-based cell manipulation is performed by directing light within the second wavelength range toward adherent cells in the cell culture containers. The directed light within the second wavelength range is at least partially absorbed by the semi-transparent surface, such that the adherent cells are selectively lysed within the cell culture containers. This links wavelength-selective imaging and manipulation through the permanently attached semi-transparent surface.
The system also includes a computing subsystem communicatively coupled to the cell culture process modules. The computing subsystem processes images of the cell culture processes and manages the cell culture processes based at least in part on the image processing, and managing comprises controlling the cell culture process modules to manipulate the cell culture processes. The documented implementations emphasize modularity and image-based control in the cell culture container format.
Claims Coverage
The document includes two independent claims, a system claim and a method claim. Across these independent claims, there are three main inventive feature areas centered on wavelength-selective imaging and manipulation through a permanently attached semi-transparent surface, selective lysis of adherent cells via absorption by that surface, and image-processing-based management by a computing subsystem.
Semi-transparent surface enables wavelength-selective imaging and manipulation
Each cell culture container comprises at least one semi-transparent surface permanently attached thereto, configured to enable light-based cell imaging when illuminated with light within a first wavelength range and light-based cell manipulation when illuminated with light within a second wavelength range.
Selectively lyse adherent cells by directing second-wavelength light absorbed by the semi-transparent surface
Light within the second wavelength range is directed toward adherent cells in the cell culture containers, and the directed light is at least partially absorbed by the semi-transparent surface to selectively lyse the adherent cells.
Image processing by a computing subsystem used to manage and control cell culture processes
A computing subsystem communicatively coupled to the cell culture process modules processes images of the cell culture processes and manages the cell culture processes based at least in part on the image processing, wherein managing comprises controlling the cell culture process modules to manipulate the cell culture processes.
Overall, the independent claims cover a modular bioprocessing system and method that combine wavelength-selective imaging and manipulation through a permanently attached semi-transparent surface, selective lysis of adherent cells via absorption by that surface, and image-processing-based control of the modules by a computing subsystem.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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