Compositions comprising recombinant Bacillus cells and an insecticide
Inventors
CURTIS, Damian • Thompson, Brian
Assignees
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Publication Number
US-11856956-B2
Publication Date
2024-01-02
Expiration Date
Abstract
The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
Core Innovation
The invention provides recombinant exosporium-producing Bacillus cells that express a fusion protein comprising an endoglucanase having at least 90% sequence identity to SEQ ID NO: 107 and an exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence. The recombinant Bacillus cells are derived from Bacillus thuringiensis BT013A, deposited under Agricultural Research Service Culture Collection Accession NRRL No. 50924.
The invention also provides compositions combining the recombinant exosporium-producing Bacillus cells with at least one insecticide selected from a specified group. The combination is provided in a synergistically effective amount.
A related aspect treats a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health. The method comprises simultaneously or sequentially applying the recombinant exosporium-producing Bacillus cells expressing the fusion protein together with at least one insecticide in a synergistically effective amount.
Claims Coverage
The document includes two independent claims covering a composition and a method of treating plants. Across these independent claims, there are five main inventive features.
Recombinant exosporium-producing Bacillus cells expressing an endoglucanase fusion protein
Recombinant exosporium-producing Bacillus cells that express a fusion protein comprising an endoglucanase with at least 90% sequence identity to SEQ ID NO: 107.
Exosporium targeting sequence in the fusion protein
An exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence.
Synergistically effective amount of at least one specified insecticide
At least one insecticide selected from the group consisting of the enumerated insecticides, wherein the insecticide is in a synergistically effective amount.
Bacillus thuringiensis BT013A-derived recombinant cells
The recombinant Bacillus cells are derived from Bacillus thuringiensis BT013A, wherein strain BT013A has been deposited under Agricultural Research Service Culture Collection Accession NRRL No. 50924.
Simultaneously or sequentially applying the recombinant cells and insecticide to treat plants
A method of treating a plant, a plant part, or the locus surrounding the plant by simultaneously or sequentially applying the recombinant exosporium-producing Bacillus cells expressing the fusion protein together with at least one selected insecticide in a synergistically effective amount.
Overall, the claim coverage centers on recombinant exosporium-producing Bacillus cells expressing a fusion protein with an endoglucanase (≥90% identity to SEQ ID NO: 107) and an exosporium targeting sequence/protein/fragment, combined with at least one enumerated insecticide in a synergistically effective amount.
Stated Advantages
Enhance plant growth and/or promote plant health.
Protects plants and plant parts.
Improves harvest yield and/or harvest quality.
Controls a very broad spectrum of agricultural pests including insects, mites, nematodes, molluscs, and phytoparasitic pests.
Is well tolerated by plants and by the environment.
May yield synergistic or super-additive effects.
Documented Applications
Treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health by simultaneously or sequentially applying recombinant exosporium-producing Bacillus cells expressing a fusion protein together with at least one insecticide in a synergistically effective amount.
Seed treatment using seed-dressing formulations comprising the described components, including use on a wide range of plant/seed types, including transgenic plants.
Agricultural pest control targeting phytoparasitic pests across broad pest groups including Arthropoda (Insecta, Arachnida), Collembola, Diplopoda, Mollusca, and Nematoda.
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