Polymer and device for capturing or separating leucocytes, manufacturing method and use thereof
Inventors
Chang, Yung • Yeh, Chih-Chen • Yang, Cheng-Chen • Jhong, Jheng-Fong
Assignees
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Publication Number
US-11833289-B2
Publication Date
2023-12-05
Expiration Date
Abstract
Disclosed is a polymer for capturing or separating leukocytes. The polymer is prepared by a polymerization reaction of monomers containing an amino and a hydroxyl. The monomer containing an amino and a hydroxyl has the structure of formula (1): In formula (1), R1 is independently selected from the group consisting of a hydrogen, a methyl, an ethyl, a hydroxyl, any one of C1 to C12 long carbon chains, and a benzene ring, R2 is independently selected from the group consisting of a hydrogen, a methyl, an ethyl, any one of from C1 to C6 long carbon chains, an amino and a benzene ring, and n is an integer of 1 to 5.
Core Innovation
The invention relates to a blood-treatment device and a method for blood treatment that use a leukocyte-capturing polymer. The polymer consists essentially of an amide-hydroxyl-containing monomer and an other monomer, where the other monomer is an anchoring unit or a crosslinking agent, and the polymer is prepared by polymerizing the amide-hydroxyl-containing monomer with the other monomer.
The amide-hydroxyl-containing monomer has a structure of formula (1), and the polymer includes repeat units having an amide group and a hydroxyl group from the amide-hydroxyl-containing monomer. The repeat unit specifically adsorbs leukocytes through the —NH— group and the —OH group originating from the amide-hydroxyl-containing monomer.
The polymer is disposed on a substrate within a housing, so the substrate together with the polymer layer forms a body for capturing or separating leukocytes. A blood sample comprising leukocytes is passed through the device such that the repeat unit specifically adsorbs the leukocytes, enabling capturing or separating leukocytes from blood.
Claims Coverage
The document includes two independent claims. Across the two independent claims, the core coverage is directed to a polymer consisting essentially of an amide-hydroxyl-containing monomer plus an anchoring unit or crosslinking agent, with repeat units configured to specifically adsorb leukocytes via —NH— and —OH groups, used in a blood-treatment method or a device for capturing or separating leukocytes.
Leukocyte-adsorbing polymer consisting essentially of an amide-hydroxyl-containing monomer and an anchoring unit or crosslinking agent
A polymer consisting essentially of an amide-hydroxyl-containing monomer and an other monomer, wherein the other monomer is an anchoring unit or a crosslinking agent, and the polymer is prepared by polymerizing the amide-hydroxyl-containing monomer with the other monomer.
Amide-hydroxyl-containing monomer repeat unit configured to specifically adsorb leukocytes
The amide-hydroxyl-containing monomer has a structure of formula (1), and a repeat unit of the polymer has an amide group and a hydroxyl group from the amide-hydroxyl-containing monomer, where the repeat unit is configured to specifically adsorb leukocytes.
Blood-treatment method by passing leukocyte-containing blood through the device
A method comprising providing a device for blood treatment with the polymer, providing a blood sample comprising leukocytes, and passing the blood sample through the device so that the repeat unit of the polymer specifically adsorbs the leukocytes.
Device with housing and polymer disposed on a substrate for capturing or separating leukocytes
A device for capturing or separating leukocytes comprising a housing and a body in the housing, where the body comprises a substrate and a polymer disposed on the substrate, the polymer consisting essentially of an amide-hydroxyl-containing monomer and an other monomer, prepared by polymerized reaction using the amide-hydroxyl-containing monomer and the other monomer, with a repeat unit configured to specifically adsorb leukocytes.
Overall, the claim coverage is anchored on a polymer consisting essentially of an amide-hydroxyl-containing monomer together with an anchoring unit or crosslinking agent, and on repeat units having —NH— and —OH groups configured to specifically adsorb leukocytes, implemented either as a method or as a device structure with a housing, substrate, and polymer layer.
Stated Advantages
Leukocyte capture rate of ≥70%.
Leukocyte capture rate of ≥87%.
Platelet retention rate of ≥85%.
Documented Applications
Leukocyte depletion in a blood treatment context by passing a leukocyte-containing blood sample through a leukocyte-capturing/separating device.
Leukocyte capture or separation using a device that includes a housing and a polymer disposed on a substrate to specifically adsorb leukocytes.
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