Agricultural compositions and related methods
Inventors
VAN ROOIJEN, Maria Helena Christine • TAM, Hok Hei • Martin, Barry Andrew • CABANILLAS, DANIEL GARCIA • SCHWIZER, SIMON • NUKOLOVA, NATALIYA VLADIMIROVNA • Niu, Yajie
Assignees
Flagship Pioneering Inc • Flagship Pioneering Innovations VI Inc
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Publication Number
US-11827897-B2
Publication Date
2023-11-28
Expiration Date
Abstract
Disclosed herein are agricultural compositions comprising plant messenger packs (PMPs), methods for using the same, and methods and related bioreactors for manufacturing PMPs.
Core Innovation
The invention provides a method for producing plant messenger packs (PMPs) by culturing a plant in a culture medium in a hydroponic system comprising a chemical stimulus, harvesting the culture medium, and purifying PMPs from the culture medium. A defining limitation is that the harvesting does not comprise disruption of the cell membranes of the plant. The disclosure frames PMP production as obtaining PMPs released into the culture medium while avoiding disruption of plant cell membranes during harvesting.
The described approach focuses on recovering PMPs secreted from a root of the plant into the culture medium, with harvesting that comprises separating the culture medium from the plant. The framework further includes refinements to the culture conditions, the chemical stimulus, and hydroponic system capacity. Dependent embodiments also include PMP compositions and PMP utility, including compositions with agriculturally acceptable carriers for plant delivery and pharmaceutically acceptable carriers for administration to human.
The disclosure further includes determining PMP purity and collecting pure PMP fractions. The compositions can include heterologous functional agents, including agriculturally active agents, therapeutic agents for human delivery, and plant-modifying agents comprising at least one gRNA and a Cas endonuclease.
Claims Coverage
The claim coverage centers on producing PMPs under hydroponic culture with a chemical stimulus and purifying PMPs from harvested culture medium without disruption of plant cell membranes. The inventive features repeated across the items include membrane-intact harvesting, root secretion into the culture medium, separation of the culture medium from the plant, hydroponic system capacity constraints, and plant-modifying agent components including gRNA and a Cas endonuclease.
Hydroponic PMP production with chemical stimulus and membrane-intact harvesting
Culturing a plant in a culture medium in a hydroponic system comprising a chemical stimulus, harvesting the culture medium, and purifying PMPs from the culture medium, wherein the harvesting does not comprise disruption of the cell membranes of the plant.
Root-secreted PMPs into the culture medium
The PMPs are secreted from a root of the plant into the culture medium.
Harvesting by separating culture medium from the plant
The harvesting comprises separating the culture medium from the plant.
Hydroponic system capacity constraints
Using a hydroponic system having a capacity that is at least one of 1 L, 100 L, and 500 L.
Plant-modifying agent comprising gRNA and a Cas endonuclease
The plant-modifying agent comprises at least one gRNA and a Cas endonuclease.
Overall, the inventive coverage is centered on hydroponic culturing with a chemical stimulus, harvesting and purification of PMPs from the culture medium while preserving plant cell membranes during harvesting, and dependent refinements for secretion, separation, hydroponic capacity, and plant-modifying agent components.
Stated Advantages
Purifying PMPs from harvested culture medium without disruption of the plant cell membranes during harvesting.
Documented Applications
Using PMP compositions as biotic stimulants for plant pest contexts, including fungal diseases and viral, insect, mollusk, and nematode pests.
PMP compositions formulated with agriculturally acceptable carriers for plant delivery and pharmaceutically acceptable carriers for administration to human.
Delivery/use concepts for increasing plant fitness, including delivery to seed, pollen, protoplast, or meristematic tissue.
PMP compositions with categories of plant pest and plant-modifying contexts, including antifungal, antibacterial, insecticidal, nematicidal, molluscicidal, virucidal, herbicidal, repellent, fertilizing, and plant-modifying agents.
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