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Publication Number
US-11821898-B2
Publication Date
2023-11-21
Expiration Date
Abstract
The subject matter disclosed herein provides a method and a device for detection of one or more bacteria in a sample.
Core Innovation
The invention concerns a rapid lateral-flow immunoassay for Lyme disease (QuickLyme), including a competitive detection format using VlsE/C6 peptides and streptavidin-gold with test and control lines on a nitrocellulose strip. Capillary flow occurs into a cellulose wick to carry assay components and enable the color readout at the lines.
The document describes the assay as intended for tick samples and provides exemplar positive/negative readout behavior. It also describes use of nitrocellulose strips pre-loaded with VlsE C6 peptide-specific antibody and biotinylated albumin/streptavidin-gold to support competitive binding and line-based detection.
For device implementation, the document outlines a device fabrication concept using a holder with glass slides, a sample pad, a nitrocellulose strip disposed between the slides, and a cellulose wick contacting an edge of the nitrocellulose strip. The holder includes openings to receive a pestle for homogenization of a sample on the sample pad while constraining movement during homogenization.
Claims Coverage
The partial content includes one independent claim (clm-00001). The inventive features cover a Borrelia-detection device architecture with a holder and enclosed slides, integrated lateral-flow components, and competitive binding reagents pre-loaded on distinct portions of an elongated nitrocellulose strip, plus a sample opening shaped for homogenization.
Holder with sample and observation openings for constrained homogenization
A device including a holder with a top part comprising at least one sample opening and at least one observation opening and a continuous bottom part, where the at least one sample opening is shaped to receive a pestle for homogenization of the sample on the sample pad and is configured to constrain movement of the sample on the sample pad during homogenization following application to the sample pad.
Encased top and bottom glass slides
A top glass slide and a bottom glass slide are disposed between the top part and the bottom part of said holder such that the holder encases the top glass slide and the bottom glass slide.
Sample pad aligned to openings and contacting a short edge of an elongated nitrocellulose strip
At least one sample pad is disposed on top of the bottom glass slide such that at least a portion of the at least one sample pad is not covered by the top glass slide, is in contact with a first short edge of at least one elongated nitrocellulose strip, and at least a portion of the sample pad aligns with at least one sample opening on the top part of the holder.
Nitrocellulose strip pre-loaded with VlsE C6 peptide-specific antibody and biotinylated streptavidin
A first portion of the at least one nitrocellulose strip is pre-loaded with a variable major protein (VMP)-like sequence (Vls) E lipoprotein (VlsE) C6 peptide-specific antibody and a second portion of the at least one nitrocellulose strip is pre-loaded with biotinylated streptavidin.
Cellulose wick contacting a second short edge of the nitrocellulose strip
At least one cellulose wick is disposed in contact with a second short edge of the at least one nitrocellulose strip, with the at least one nitrocellulose strip disposed between the top glass slide and the bottom glass slide.
Across the independent claim, the device is characterized by a holder with sample and observation openings, a layered glass-slide enclosure, a sample pad positioned to contact an edge of an elongated nitrocellulose strip, distinct pre-loaded portions for VlsE C6 peptide-specific antibody and biotinylated streptavidin, and a cellulose wick contacting a second edge of the nitrocellulose strip.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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