Microfluidic system and method with focused energy apparatus
Inventors
Appleyard, David • BETTHAUSER, Jeff • FAUST, Marjorie • Larsen, John • SHAO, Guocheng • Xia, Zheng • Zhou, Yu
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11796449-B2
Publication Date
2023-10-24
Expiration Date
Abstract
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
Core Innovation
The invention relates to a method of producing a sperm cell composition by flowing a stream of a population of sperm cells through a channel and differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells. A desired subpopulation is selected, and an undesired subpopulation is ablated at a predetermined delay time from selecting the desired subpopulation, wherein the predetermined delay time is 1 μs or longer. Both subpopulations are collected together.
The invention is also directed to producing at least one fertilized egg from a female mammal by flowing a stream of a population of sperm cells through a channel and differentiating the sperm cells into two X-chromosome containing and Y-chromosome containing subpopulations. After selecting a desired subpopulation, the undesired subpopulation is ablated at a predetermined delay time of 1 μs or longer, both subpopulations are collected together, and at least a portion of the collected sperm cells is mixed with at least one egg from the female mammal to produce a fertilized egg.
A further embodiment produces at least one embryo from a female mammal by flowing a sperm-cell stream through a channel and differentiating sperm cells into X-chromosome containing and Y-chromosome containing subpopulations, selecting a desired subpopulation, and ablating the undesired subpopulation after a predetermined delay time of 1 μs or longer. Both subpopulations are collected together, and at least a portion of the collected sperm cells is inserted into a female mammal prior to fertilizing at least one egg to produce at least one embryo.
Claims Coverage
The independent claims covered by the provided list are clm-00001, clm-00040, and clm-00043, each sharing a common core structure with three inventive features: differentiating sperm into X- and Y-chromosome subpopulations with selection and delayed ablation, collecting both subpopulations together, and downstream use for sperm-cell composition, fertilized egg production, or embryo production.
X/Y-chromosome subpopulation differentiation and selection with delayed ablation
Flowing a stream of a population of sperm cells through a channel; differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells; selecting a desired subpopulation; ablating an undesired subpopulation at a predetermined delay time from selecting the desired subpopulation, wherein the predetermined delay time is 1 μs or longer; and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together.
Fertilized egg production using collected desired and ablated undesired sperm
Flowing a stream of a population of sperm cells through a channel; differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells; selecting a desired subpopulation; ablating an undesired subpopulation at a predetermined delay time from selecting the desired subpopulation, wherein the predetermined delay time is 1 μs or longer; collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together; and mixing at least a portion of the collected sperm cells with at least one egg from a female mammal to produce a fertilized egg.
Embryo production with collected desired and ablated undesired sperm insertion and fertilization
Flowing a stream of a population of sperm cells through a channel; differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells; selecting a desired subpopulation; ablating an undesired subpopulation at a predetermined delay time from selecting the desired subpopulation, wherein the predetermined delay time is 1 μs or longer; collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together; inserting at least a portion of the collected sperm cells into a female mammal; and fertilizing at least one egg from said female mammal to produce at least one embryo from said female mammal.
Across clm-00001, clm-00040, and clm-00043, the claim coverage is centered on producing a composition of sperm that includes a desired X- or Y-chromosome-containing subpopulation while ablating the undesired subpopulation at a predetermined delay time of 1 μs or longer, then collecting both subpopulations together. The downstream inventive coverage is directed to mixing collected sperm with at least one egg to produce fertilized eggs, and/or inserting collected sperm into a female mammal and fertilizing eggs to produce embryos.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
Interested in licensing this patent?