Protein hydrolysates and methods of making same
Inventors
Lambers, Teartse Tim • Bommel, Cindy van • Ao, Zihua • Wittke, Anja • Dingess, Kelly Alina • Hettinga, Kasper
Assignees
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Publication Number
US-11785976-B2
Publication Date
2023-10-17
Expiration Date
Abstract
The present disclosure generally relates to methods of preparing protein hydrolysates for use in pediatric nutritional compositions. Specifically, alpha-, beta- and/or kappa casein enriched casein, acid casein or caseinates can be hydrolyzed with proteases such as trypsin (trypsin-like), chymotrypsin (chymotrypsin-like), pepsin and/or plasmin to produce a hydrolysate that is close to the peptide composition of human breast milk.
Core Innovation
The invention relates to nutritional compositions for pediatric nutrition that include a milk protein preparation hydrolyzed into a protein hydrolysate. The milk protein preparation includes casein, acid casein, or caseinate as starting materials, and the resulting protein hydrolysate comprises alpha-casein peptides, beta-casein peptides, and kappa-casein peptides.
The protein hydrolysate is produced by hydrolyzing the milk protein preparation with at least two proteases selected from trypsin, proteolytic enzymes that cleave peptide bonds following a positively charged amino acid, chymotrypsin, proteolytic enzymes that cleave peptide bonds following a tyrosine, phenylalanine or tryptophan residue, pepsin, and plasmin. The use of proteases is described to generate peptide compositions intended to be closer to the human breast milk peptidome.
The nutritional compositions further specify peptide composition targets, including that beta-casein peptides comprise about 25% to about 60% of the total peptides, and that kappa-casein peptides comprise about 1% to about 10% of the total peptides. Additional peptide fractions derived from named biological components are also described for inclusion, including hydrolysates of polymeric immunoglobulin receptor (PIGR), osteopontin, bile-salt activated lipase, and clusterin.
Claims Coverage
The partial content shows two independent claims. One inventive structure is a casein, acid casein, or caseinate-derived protein hydrolysate produced with at least two specified proteases and defined beta- and kappa-casein peptide proportion ranges; the other is a protein hydrolysate composition defined primarily by beta- and kappa-casein peptide proportions.
Hydrolyzed casein with multiple specified proteases to form mixed casein peptide fractions
A nutritional composition comprising a milk protein preparation selected from casein, acid casein or caseinate, wherein the milk protein preparation is hydrolyzed with at least two proteases selected from trypsin, proteolytic enzymes that cleave peptide bonds following a positively charged amino acid, chymotrypsin, proteolytic enzymes that cleave peptide bonds following a tyrosine, phenylalanine or tryptophan residue, pepsin, and plasmin to form a protein hydrolysate comprising alpha-casein peptides, beta-casein peptides, and kappa-casein peptides.
Beta- and kappa-casein peptide proportion targets in the nutritional composition
A nutritional composition wherein beta-casein peptides in the protein hydrolysate comprise about 25% to about 60% of the total peptides, and wherein kappa-casein peptides in the protein hydrolysate comprise about 1% to about 10% of the total peptides.
Protein hydrolysate defined by beta- and kappa-casein peptide proportions
A nutritional composition comprising a protein hydrolysate comprising alpha-casein peptides, beta-casein peptides, and kappa-casein peptides, wherein beta-casein peptides comprise about 25% to about 60% of the total peptides, and wherein kappa-casein peptides comprise about 1% to about 10% of the total peptides.
The claim coverage is centered on nutritional compositions containing a protein hydrolysate with defined alpha-casein, beta-casein, and kappa-casein peptide fractions, with beta-casein and kappa-casein peptide proportions constrained to about 25% to 60% and about 1% to 10%, respectively; one independent claim further requires hydrolysis using at least two of the listed proteases.
Stated Advantages
Documented Applications
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