Construct including an anchor, an enzyme recognition site and an indicator region for detecting microbial infection in wounds

Inventors

GUSE, Jan Hinrich • Reisser, Martin • Pietrzik, Nikolas • Baeuerlein, Christiane • Eitel, Kornelia

Assignees

Synovo GmbH

Abstract

The disclosed technology relates to chemical entities for the detection of wounds, e.g., chronic wounds or infected wounds, including compositions, substrates, kits, dressing materials, and articles, and systems containing such compounds. The disclosed technology further relates to methods of using these compositions, kits and systems in diagnostic assays, and in the diagnosis and/or detection of chronic or infected wounds based on enzymatic action on specific moieties and/or reaction sites. The disclosed technology additionally relates to detection of pathogenic, e.g., bacterial and/or viral substances, such as enzymes and substrates, at the wound situs. Additional disclosure relates to methods of characterizing wounds based on expression of a plurality of markers and using such information to treat, manage, and follow-up patients suffering from chronic or infected wounds.

Core Innovation

The invention relates to chemical entities of Formula I comprising an anchor (A), an enzyme recognition site (R), and an indicator region (I). The anchor (A) comprises a fluorenylmethyloxycarbonyl group (Fmoc) linked to the N-terminus of the peptide, and the enzyme recognition site (R) comprises a peptide of from four to eight amino acids having an N-terminus and a carboxy terminus, with at least one enzyme-labile region.

The indicator region (I) comprises an alcohol-containing or amine-containing chromophore or chromophore precursor linked via an amide, ester, or carbonate bond to the carboxy terminus of the peptide. Further embodiments specify amino acid sequence patterns containing variable amino acids (X), variable repeat parameter y, and optional linking moiety (L) together with a label (Z) selected from indoxyl compounds, indole, naphthol, Fast Blue RR, and Remazol Brilliant Blue.

The document further describes multiplex chemical entities containing multiple enzyme recognition or reaction sites for simultaneous detection of multiple wound enzymes or markers, together with compositions, systems, kits, surfaces, and wound-dressing formats comprising Formula I compounds. It also describes support-material anchoring and immobilization concepts and solid supports for dye immobilization, including Sephabeads, Sephadex beads, epoxy-activated beads, silica gel, and Chemicell SiCore Amin beads.

Claims Coverage

Two independent claims define Formula I chemical entities built from an anchor (A), an enzyme recognition site (R), and an indicator region (I). Across the claims, the inventive features include an Fmoc-linked peptide anchor, an enzyme-labile peptide recognition site, and an indicator region chromophore or chromophore precursor linked to the peptide carboxy terminus, with further sequence and label constraints.

The claims center on Formula I compounds with an Fmoc anchor, an enzyme-labile peptide recognition site, and a chromophore-based indicator region linked through amide, ester, or carbonate bond chemistry, with further sequence parameters and selected indicator labels.

Stated Advantages

Documented Applications