Methods and devices for detection of multiple analytes from a biological sample
Inventors
Krishnan, Rajaram • HINESTROSA SALAZAR, Juan Pablo • TURNER, Robert Paul • SEARSON, David Joseph • MADSEN, James Gregory • Kovelman, Robert
Assignees
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Publication Number
US-11731132-B2
Publication Date
2023-08-22
Expiration Date
Abstract
The present invention includes methods, devices and systems for isolating, identifying, analyzing, and quantifying biological materials from fluid samples. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity biological materials from complex fluids such as blood, serum, or plasma.
Core Innovation
The invention provides a method for analyzing a biological sample by capturing a plurality of analytes using an electrode configured to generate an AC dielectrophoretic field. The plurality of analytes comprises at least two types selected from DNA, RNA, nucleosomes, exosomes, extracellular vesicles, proteins, cell membrane fragments, mitochondria and cellular vesicles.
The method addresses capturing and detection of multiple analyte classes from a biological sample using an electrode-based dielectrophoretic approach that generates an AC dielectrophoretic field. The method supports preferential capture of different analytes using separate electrodes, where a first electrode captures a first analyte and a second electrode captures a second analyte.
Detection further supports binding-based analyte identification using an antibody that specifically binds to the analyte, and one or more selected detection techniques from a listed group, including quantitative Real Time PCR, enzyme-linked immunosorbent assay (ELISA), direct SYBR gold assay, direct PicoGreen assay, loss of heterozygosity (LOH) of microsatellite marker assay, electrophoresis, methylation analysis, MALDI-ToF, PCR and digital PCR. The method can also detect and determine cancer-related conditions such as cancer type, cancer stage, tumor burden change states, therapy efficacy, or whether cancer is absent.
Claims Coverage
Independent claim 1 covers capturing a plurality of analytes under an AC dielectrophoretic field and detecting at least two different species among specified analyte categories, i.e., it includes 1 independent claim with 1 core inventive concept.
Capturing plurality of analytes using an AC dielectrophoretic electrode field
Capturing a plurality of analytes in the biological sample using an electrode configured to generate an AC dielectrophoretic field, wherein the plurality of analytes comprises at least two types selected from DNA, RNA, nucleosomes, exosomes, extracellular vesicles, proteins, cell membrane fragments, mitochondria and cellular vesicles.
Detecting at least two different analyte species
Detecting the plurality of analytes, wherein detecting the plurality of analytes comprises detecting at least two different species selected from DNA, RNA, nucleosomes, exosomes, extracellular vesicles, proteins, cell membrane fragments, mitochondria and cellular vesicles.
The claims coverage is centered on electrode-based capture of multiple analyte types using an AC dielectrophoretic field, combined with detection of at least two different species within the specified analyte categories. Dependent claim features further specify preferential capture using separate electrodes, antibody-based detection, selectable detection techniques, and cancer-related interpretation outputs.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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