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Publication Number
US-11718656-B2
Publication Date
2023-08-08
Expiration Date
Abstract
The disclosure provides inducible caspase polypeptides, compositions comprising inducible caspase polypeptides and sequences encoding the same, cells modified to express the polypeptides and compositions of the disclosure, as well as methods of making and methods of using same for adoptive cell therapy.
Core Innovation
The invention relates to polynucleotides that include a nucleic acid sequence designated as SEQ ID NO: 10, and embodiments in which this sequence is used within transposon contexts. The disclosed content focuses on transposase enzyme systems, including piggyBac and Super piggyBac variants, with related sequence identifiers, and also includes Sleeping Beauty, Helraiser-related motifs, and Tol2 transposase/transposon sequences.
In the disclosed embodiments tied to the claim set, the transposon includes the SEQ ID NO: 10 polynucleotide and may encode a therapeutic protein. The therapeutic protein can be an antigen receptor, including configurations limited to TCR or CAR, and can otherwise be a variety of protein types within combinations.
Claims Coverage
The independent claim covers one inventive feature centered on a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 10. Additional claim coverage incorporates the SEQ ID NO: 10 polynucleotide into a transposon and extends to transposon-encoded therapeutic payloads and specified cell contexts.
Polynucleotide defined by SEQ ID NO: 10
A polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 10.
Transposon containing the SEQ ID NO: 10 polynucleotide
A transposon that includes the polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 10.
Therapeutic protein encoded by the transposon
The transposon additionally includes a nucleic acid sequence that encodes a therapeutic protein.
Enumerated therapeutic protein types
The transposon encodes a therapeutic protein selected from an antigen receptor, a cell surface protein, a membrane-bound protein, an intracellular, nuclear, cytoplasmic, cytosolic protein, or a secreted, lysosomal, endosomal, vesicle-associated, mitochondrial, endoplasmic reticulum, cytoskeletal protein, including proteins involved in intracellular signaling or extracellular signaling, or any combination thereof.
Antigen receptor limited to TCR or CAR
The antigen receptor is either a T-Cell Receptor (TCR) or a Chimeric Antigen Receptor (CAR).
Cell context for the polynucleotide-containing transposon
A claimed cell is a human cell, an immune cell, an artificial antigen presenting cell, a stem cell, or any combination of these types.
Overall, the claim coverage centers on a SEQ ID NO: 10 polynucleotide and its incorporation into a transposon, with downstream encoding of a therapeutic protein. The coverage further narrows to enumerated therapeutic protein categories and allows antigen receptors specifically limited to TCR or CAR, in specified human and immune-related cell contexts.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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