Hybrid-type multi-lamellar nanostructure of epidermal growth factor and liposome and method for manufacturing same
Inventors
Nam, Yoon Sung • JO, Sung Duk • KOO, Bon Il
Assignees
Korea Advanced Institute of Science and Technology KAIST • Celltrion Inc
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Publication Number
US-11712407-B2
Publication Date
2023-08-01
Expiration Date
Abstract
The present invention relates to a hybrid-type multi-lamellar nanostructure of an epidermal growth factor and a liposome and a method for manufacturing same. The new type of hybrid-type multi-lamellar nanostructure not only has a high epidermal growth factor encapsulating efficiency, but also can be manufactured through a simple process, such that the same can be easily delivered into a living body or a cell while maintaining a high physiological activity of the epidermal growth factor.
Core Innovation
The invention relates to a cosmetic method in which nanostructures are applied to skin. Each nanostructure includes a unilamellar liposome having an empty internal space composed of a cationic lipid bilayer comprising 1,2-dioleoyl-3-trimethylammonium-propane, and one or more cationic lipid bilayers cover the unilamellar liposome with encapsulated epidermal growth factor positioned between the cationic lipid bilayers.
The nanostructure is characterized by substantially spherical shells formed of lipid bilayers interspersed with aqueous layers, a particle size of 100–200 nm, and a zeta potential of +1 to 40 mV. The encapsulated epidermal growth factor is 60% or more of total epidermal growth factor composed of the encapsulated epidermal growth factor and non-encapsulated epidermal growth factor.
The method specifies a weight ratio (w/w) of total epidermal growth factor to the cationic lipid of 0.001 to 2:1, and requires allowing sufficient time for the plurality of nanostructures to permeate from the cosmetic composition to the dermis of the skin. The partial content also describes a hybrid-type EGF–cationic-lipid, multi-lamellar nanostructure formed by spontaneous self-assembly, supported by cryo-TEM and characterization of stability by circular dichroism.
Claims Coverage
The partial content provides one independent claim directed to a cosmetic method using specified EGF-loaded cationic-lipid nanostructures and requiring permeation to dermis. The independent claim includes five inventive features, and dependent claims further refine the cosmetic composition and ingredient categories.
Egf-loaded cationic-lipid multi-lamellar unilamellar nanostructure
Nanostructures dispersed in a cosmetic composition, each nanostructure comprising a unilamellar liposome having an empty internal space composed of a cationic lipid bilayer comprising 1,2-dioleoyl-3-trimethylammonium-propane; one or more cationic lipid bilayers covering the unilamellar liposome; and encapsulated epidermal growth factor between the cationic lipid bilayers.
Substantially spherical lipid-bilayer shells with defined particle size and zeta potential
The nanostructure has a series of substantially spherical shells formed of lipid bilayers interspersed with aqueous layers with a particle size of 100–200 nm and a zeta potential of +1 to 40 mV.
High encapsulation proportion of EGF
The encapsulated epidermal growth factor is 60% or more of total epidermal growth factor composed of the encapsulated epidermal growth factor and the non-encapsulated epidermal growth factor.
Specified EGF-to-cationic lipid weight ratio
A weight ratio (w/w) of the total epidermal growth factor to the cationic lipid is 0.001 to 2:1.
Dermal permeation by allowing sufficient time
Allowing sufficient time for the plurality of nanostructures to permeate from the cosmetic composition to dermis of the skin.
Overall, the claim coverage centers on applying defined EGF-loaded, cationic-lipid nanostructures with substantially spherical multi-lamellar shells, specified particle size and zeta potential, an encapsulation proportion of 60% or more, a total EGF-to-cationic lipid weight ratio of 0.001 to 2:1, and sufficient time to enable permeation from the cosmetic composition to the dermis.
Stated Advantages
Enhanced skin permeation to the dermis with the hybrid nanostructures compared with epidermal growth factor or the cationic lipid alone.
Encapsulated epidermal growth factor is delivered as 60% or more of total epidermal growth factor.
Documented Applications
Cosmetic method using the described nanostructures dispersed in a cosmetic composition and applying them to skin to allow permeation to dermis, including cosmetic formulations such as skin lotion, milk lotion, essence, nourishing cream, and pack.
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