Platforms and systems for automated cell culture
Inventors
Wagner, Matthias • Aivio, Suvi • AMENDUNI, Mariangela • PILSMAKER, Catherine • Pereira, Arnaldo • ZUTSHI, Ananya • TOURE, Anthia • Nagle, Steven • Whiting, Ozge • Harb, George • Sullivan, Matthew • BERLIN-UDI, Maya • Morgan, Stefanie • SEAY, Nick • Lee, Sang • LURO, Scott
Assignees
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Publication Number
US-11708563-B2
Publication Date
2023-07-25
Expiration Date
Abstract
Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.
Core Innovation
The invention describes an imaging system for a cell culture sample comprising one or more cells adherent to a surface of a cell culture chamber. A light source illuminates the sample, an objective captures light passing through the cell culture sample, and a sensor acquires a plurality of images based on the captured light.
During acquisition of each of the plurality of images, continuous movement is performed by moving the cell culture chamber relative to the light source and the objective or by moving the light source and the objective relative to the cell culture chamber. A computer processor generates quantitative phase images of the cell culture sample based at least in part on the acquired plurality of images.
The described workflows integrate quantitative phase imaging with continuous imaging and tracking to support monitoring and selection of adherent cell colonies while reducing cross-contamination. The document further describes quantitative phase images within a closed cassette system and modular bioprocessing concepts that include automated monitoring and editing decisions driven by computational analysis of acquired image data.
Claims Coverage
The independent claims cover 3 main inventive features centered on illumination and sensor acquisition, continuous movement during acquisition, and computer-processor generation of quantitative phase images.
Illumination, objective capture, and sensor acquisition for adherent cells
The cell culture sample comprises one or more cells adherent to a surface of the cell culture chamber, and the system illuminates the cell culture sample, captures light with an objective passing through the sample, and acquires a plurality of images with a sensor based on the captured light.
Continuous relative movement during image acquisition
An actuator performs continuous movement of the cell culture chamber relative to the light source and the objective or of the light source and the objective relative to the cell culture chamber, during acquisition of each of the plurality of images.
Processor generation of quantitative phase images from a plurality of images
A computer processor generates quantitative phase images of the cell culture sample based at least in part on the acquired plurality of images.
The independent claims are directed to producing quantitative phase images of an adherent cell culture sample by combining illumination, objective-based light capture, sensor acquisition of multiple images during continuous relative movement, and computer-processor generation of quantitative phase images from the acquired image set.
Stated Advantages
Enables monitoring and selection of adherent cell colonies while reducing cross-contamination.
Documented Applications
No documented applications found
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