Concealing information present within nucleic acids
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Publication Number
US-11667951-B2
Publication Date
2023-06-06
Expiration Date
Abstract
Methods related to concealment of genetic information present within nucleic acid sequences, wherein individual nucleic acid molecules are barcoded. In some embodiments barcoding occurs before, after, or during enrichment. Barcoded nucleic acids are then combined with control barcoded nucleic acids. Different methods are provided for barcoding and pooling to conceal different types of genetic information present within nucleic acids.
Core Innovation
Disclosed nucleic-acid concealment by barcoding individual nucleic-acid molecules. The method generates a set of barcode oligonucleotides and affixes a subset of barcode oligonucleotides to a 5′- or 3′-end of target nucleic acids to create a set of barcoded target nucleic acids.
The barcoded target nucleic acids are concealed by combining them into a mixture with one or more barcoded decoy nucleic acids. The barcoded decoy nucleic acids contain a unique subset of barcode oligonucleotides different from the subset affixed to the target nucleic acids, so barcode-subset membership is obscured by the presence of decoys.
A table is prepared listing the identity and sequence(s) of the subset of barcode oligonucleotides affixed to the target nucleic acids and the identity and sequences of the barcode oligonucleotides of the barcoded decoy nucleic acids. The mixture of barcoded target nucleic acids and the table are secured by sealing both in a container that indicates when a seal of the container is broken.
Claims Coverage
The partial content provides one independent claim (clm-00001). The claim covers a complete workflow for concealing target nucleic acids using barcoded subsets, pooling with decoy barcodes containing a different subset, maintaining an identity/sequence table for both target and decoy barcode subsets, and securing both the mixture and the table with a tamper-indicating container seal.
Concealing barcoded target nucleic acids using pooled decoy barcodes with a different subset
Affixing a subset of barcode oligonucleotides to a 5′- or 3′-end of target nucleic acids, creating barcoded target nucleic acids, and concealing the set by pooling with one or more barcoded decoy nucleic acids that contain a unique subset of barcode oligonucleotides different from the subset affixed to the target nucleic acids, to create a mixture of barcoded target nucleic acids and barcoded decoy nucleic acids.
Preparing a barcode subset identity and sequence table for targets and decoys
Preparing a table listing the identity and sequence(s) of the subset of barcode oligonucleotides affixed to the target nucleic acids and the identity and sequences of the barcode oligonucleotides of the barcoded decoy nucleic acids.
Securing the mixture and the table with a tamper-indicating container seal
Securing the mixture of barcoded target nucleic acids and the table by sealing both in a container that indicates when a seal of the container is broken.
Overall, the claim coverage is focused on generating and affixing barcode subsets to target nucleic acids, concealing by pooling with decoy nucleic acids carrying a different barcode subset, preparing a table that records the identities and sequences of barcode subsets for both targets and decoys, and securing both the mixture and the table in a tamper-indicating sealed container.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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