Method for use of a double-structured tissue implant for treatment of tissue
Inventors
Shortkroff, Sonya • Tarrant, Laurence J. B. • Roos, Eric J. • Smith, Robert Lane • CLAESSON, HANS P. I.
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11648102-B2
Publication Date
2023-05-16
Expiration Date
Abstract
A method for use of a double-structured tissue implant or a secondary scaffold stand-alone implant for treatment of tissue defects. The double-structured tissue implant comprising a primary scaffold and a secondary scaffold consisting of a soluble collagen solution in combination with a non-ionic surfactant generated and positioned within the primary scaffold. A method of use of a stand-alone secondary scaffold implant or unit for treatment of tissue defects.
Core Innovation
The invention relates to a double-structured tissue implant for treating a tissue defect. The implant includes a three-dimensional primary scaffold having a plurality of pores, where the pores comprise a secondary scaffold comprising lyophilized and dehydrothermally treated collagen and a surfactant, forming a fibrous and cross-linked collagen network within the plurality of pores.
The tissue implant is prepared for implantation as part of a method for treating a tissue defect. A tissue defect is prepared, the double-structured tissue implant is precut, and the defect is coated with a first tissue adhesive. The implant is implanted into the defect, rehydrated with a physiologically acceptable solution or buffer before or after implantation, and covered with a second tissue adhesive.
The collagen-based secondary scaffold is formed by introducing a soluble collagen solution with a non-ionic surfactant into the primary scaffold pores, followed by solidifying using precipitation/gelation, then washing, lyophilization, and dehydrothermal treatment to yield a fibrous, cross-linked collagen network within the pores. The implant may be implanted dry or rehydrated, and a stand-alone secondary scaffold is also described.
Claims Coverage
Independent claim 1 covers a complete method for treating a tissue defect using a double-structured tissue implant with a primary porous scaffold whose pores contain a secondary lyophilized and dehydrothermally treated collagen/surfactant scaffold forming a fibrous, cross-linked collagen network, combined with defect preparation, precutting, adhesive coating, and implant implantation with pre- or post-implantation rehydration using a physiologically acceptable solution or buffer, followed by covering with a second tissue adhesive. No other independent claims are explicitly provided in the supplied text.
Double-structured tissue implant forming a fibrous cross-linked collagen network in pores
The three-dimensional primary scaffold comprises a plurality of pores, wherein the plurality of pores comprises a secondary scaffold comprising lyophilized and dehydrothermally treated collagen and a surfactant, and wherein the secondary scaffold forms a fibrous and cross-linked collagen network within said plurality of pores.
Implantation method with adhesive coating and precutting of the double-structured tissue implant
Preparing a double-structured tissue implant comprising a three-dimensional primary scaffold; preparing said tissue defect for implantation; precutting said double-structured tissue implant; coating said defect with a first tissue adhesive; implanting said double-structured tissue implant into said defect; and covering said implant with a second tissue adhesive.
Rehydration with physiologically acceptable solution or buffer before or after implantation
Implanting said double-structured tissue implant into said defect, wherein said implant is rehydrated with a physiologically acceptable solution or buffer before or after implantation.
The core claim coverage centers on a double-structured implant in which secondary collagen that is lyophilized and dehydrothermally treated with a surfactant forms a fibrous, cross-linked collagen network inside pores of a three-dimensional primary scaffold, together with defect preparation, precutting, first and second tissue adhesive coating steps, and implantation with rehydration using a physiologically acceptable solution or buffer before or after implantation.
Stated Advantages
Improved wettability and rapid rehydration (<2 s).
High collagen retention (~99% initially).
Minimal shrinkage and swelling (about 2–5% surface area change in the first hour).
Good cell compatibility/viability (about 96–100%) with extracellular matrix deposition after 14 days.
Documented Applications
Treating a tissue defect using a method comprising implantation of a double-structured tissue implant, with first and second tissue adhesive coating and rehydration with a physiologically acceptable solution or buffer.
Implantation with optional cells and growth factors or drugs, including localization in primary scaffold versus secondary scaffold.
Use of the implant in a dry or rehydrated implantation option.
Stand-alone secondary scaffold application is described.
Interested in licensing this patent?