Compositions and methods for transport and preservation of biological material

Inventors

L'Huillier, Andrew • Bryan, Ayanna • Smithmyer, Martin

Assignees

Americord Registry LLC

Abstract

Compositions and methods for the shipping, packaging, and freezing of whole or partial biological materials including but not limited to cells, tissues, and organs, designed to simultaneously reduce freezing damage and maintain tissue structure. The compositions include a buffered hyperosmotic solution, an oncotic agent, an antioxidant, a metal chelator, and a ketone body. These aspects of the present invention are applicable to transport and preservation of biological materials including but not limited to amniotic membrane of the placenta and umbilical cord and are applicable to preservation of cellular and acellular biological materials generally.

Core Innovation

The invention provides a transport and cryogenic freezing and storage composition for biological material and live biological material. The composition includes a buffered hyperosmotic solution, an oncotic agent, an antioxidant, a metal chelator, and a ketone body, and is designed to reduce freezing/thaw damage while maintaining tissue structure.

The composition targets osmotic stability and chemical and oxidative stress through the combination of an oncotic carbohydrate, an antioxidant, and a metal chelator. The ketone body component is used to support energy metabolism with a lower reactive oxygen species contribution than glucose, while the formulation limits calcium-related damage by omitting Ca2+ to limit calcium flux.

The document further describes optional components that can be included with the base formulation, including a polymer and optional agents. The polymer is optionally polyethylene glycol, and optional additives can include DMSO, dextrose, and mannitol.

Claims Coverage

The document includes two independent claims. Across the independent claims, the main inventive features are the specific combination of buffered hyperosmotic solution, oncotic agent, antioxidant, metal chelator, and ketone body, with the second independent claim adding a polymer component for cryogenic freezing and storage.

Claim coverage centers on compositions that combine a buffered hyperosmotic solution with an oncotic agent, antioxidant, metal chelator, and ketone body, where the cryoprotectant further includes a polymer.

Stated Advantages

Documented Applications