Fluidic device and method for separating biomolecules
Inventors
Yasui, Takao • Baba, Yoshinobu
Assignees
Interested in licensing this patent?
MTEC can help explore whether this patent might be available for licensing for your application.
Publication Number
US-11571695-B2
Publication Date
2023-02-07
Expiration Date
Abstract
The present disclosure provides a device for separating biomolecules comprising a substrate having a planar surface, nanowires disposed on at least a portion of the planar surface, and a fluid chamber formed to include at least a portion of the nanowires.
Core Innovation
A fluidic device for separating a biomolecule includes a substrate having a planar surface with nanowires disposed on at least a portion of the planar surface. A cover has a bonding surface and a recess surrounded by the bonding surface, and the bonding surface is in intimate contact with a portion of the planar surface of the substrate so that the planar surface of the substrate and the recess of the cover define a fluid chamber including at least a portion of the nanowires.
The covered nanowire region forms the fluid chamber for separating a biomolecule while allowing solution introduction into the fluidic device. In some embodiments, the cover recess includes a microstructure to agitate flow of a solution, and an inlet introduces a solution into the fluid chamber and an outlet discharges a solution from the fluid chamber.
A method of separating a biomolecule includes providing the fluidic device with the substrate, nanowires, and the cover recess forming the fluid chamber, and introducing a solution comprising the biomolecule into the fluidic device. The biomolecule can include RNA or an extracellular vesicle, and the method can further include introducing a cell lysate, draining the cell lysate, and measuring nucleic acids in the drained cell lysate.
Claims Coverage
The document contains two independent claims. Across the independent claims, coverage focuses on a nanowire-enclosed fluid chamber formed by a substrate planar surface and a cover recess, and on a method of separating a biomolecule by introducing a biomolecule-containing solution into that chamber.
Nanowire-enclosed fluid chamber formed by substrate planar surface and cover recess
A substrate having a planar surface; nanowires disposed on at least a portion of the planar surface; and a cover having a bonding surface and a recess surrounded by the bonding surface, the bonding surface being in intimate contact with a portion of the planar surface of the substrate, wherein the planar surface of the substrate and the recess of the cover define a fluid chamber including at least a portion of the nanowires.
Solution introduction into the fluid chamber for separating a biomolecule
Providing a fluidic device comprising a substrate having a planar surface; nanowires disposed on at least a portion of the planar surface; a cover having a bonding surface and a recess surrounded by the bonding surface, the bonding surface being in intimate contact with a portion of the planar surface of the substrate, wherein the planar surface of the substrate and the recess of the cover define a fluid chamber including at least a portion of the nanowires; and introducing a solution comprising the biomolecule into the fluidic device.
The independent claims collectively cover a nanowire-based fluidic separation structure in which the substrate planar surface and the cover recess define a fluid chamber containing the nanowires, and a separation method that operates by introducing a biomolecule-containing solution into that chamber.
Stated Advantages
Improved yields versus centrifugation for downstream biomolecule analysis.
Documented Applications
Adsorption and FESEM observation of extracellular vesicles from urine.
Downstream biomolecule analysis including nucleic acid extraction and rapid detection.
Interested in licensing this patent?