Water-soluble prolamin, preparation thereof, and uses thereof for food-grade application including prolamin compositions combining prolamin and DNA taggants

Inventors

Mattei, Peter

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Assignees

Safetraces Inc

Member
SafeTraces
SafeTraces

SafeTraces provides technology-driven solutions for indoor air quality validation, biosafety, and supply chain traceability. The company specializes in aerosol tracing, DNA-based product tagging, rapid qPCR detection, and cloud-based analytics. Clients span healthcare, infrastructure, real estate, food, and government sectors, supporting regulatory compliance, operational safety, and risk management.

Publication Number

US-11555186-B2

Patent

Publication Date

2023-01-17

Expiration Date


Abstract

A water-soluble prolamin composition, such as a zein composition, and methods for producing the same. A method for tagging items comprising applying a plurality of non-coding DNA tags in a prolamin composition, such as a zein composition, wherein the selection of the particular taggants corresponds with a binary or nonbinary code sequence containing information about the tagged items.

Core Innovation

Water-soluble prolamin compositions are provided that include prolamin and [1,3]-propanediol. The compositions are essentially alcohol-free and are described in the context of water-dispersible zein complexes and zein nanoparticles stable in water.

The compositions are described as forming stable self-assembled nanosomal or nanoscale carriers that disperse in water. Food-safe surfactant components are used, exemplified by surfactin, and the carrier formulations may optionally include cyclodextrin and casein. Stabilizers may also be included to support stability and aqueous dispersion.

The prolamin carrier complex is described for associating non-coding DNA taggants for item and food/sanitation traceability. DNA taggant sets are described as unique combinations of multiple DNA sequences, including binary and nonbinary coding combinations, and the taggant systems are described as prepared as liquids or powders.

Claims Coverage

Two independent claims are identified: one directed to a prolamin/[1,3]-propanediol composition and one directed to a method of preparing that composition. The inventive features focus on the defined composition components, the preparation workflow, and optional refinements including zein nanoparticles, essentially ethanol-free formulation, and DNA taggant sets with unique combinations of multiple DNA sequences.

Prolamin and [1,3]-propanediol composition

A composition comprising prolamin and [1,3]-propanediol.

Prolamin/[1,3]-propanediol mixed with water to prepare the composition

A method of preparing a composition comprising obtaining a first solution comprising prolamin and [1,3]-propanediol, obtaining a second solution comprising water, and mixing the first solution with the second solution to prepare the composition.

The claim coverage centers on a composition defined by prolamin together with [1,3]-propanediol and a preparation method defined by forming a prolamin/[1,3]-propanediol first solution, forming a water second solution, and mixing them to obtain the composition. Additional described claim refinements include zein or zein nanoparticles, essentially ethanol-free composition, powder form, and DNA taggant sets defined by a unique combination of multiple DNA sequences.

Stated Advantages

Improved aqueous dispersion.

Reduced aggregation.

Improved DNA stability.

Elimination of flammable alcohol processing.

Documented Applications

Association of non-coding DNA taggants for item and food/sanitation traceability, using unique combinations of DNA sequences in binary or nonbinary encoding.

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