Drug detection via surface enhanced Raman spectroscopy
Inventors
Stadthagen, Torsten • FREMMER, Markus
Assignees
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Publication Number
US-11511273-B2
Publication Date
2022-11-29
Expiration Date
Abstract
The present invention relates to a method for determining an analyte using surface enhanced RAMAN spectroscopy and to a device which is suitable for this purpose.
Core Innovation
The invention relates to a kit and a method for determining an analyte in a sample, wherein the analyte is detected via surface enhanced Raman spectroscopy (SERS) in an analysis device. The kit includes a sampling device configured for taking up a sample containing an analyte, and the sampling device comprises a sample matrix. The analysis device includes a first region configured for introducing the sampling device and a second region configured for detecting the presence and/or amount of the analyte via SERS.
A processing stage is located downstream from the sample matrix with respect to an intended flow direction of a fluid, and the processing stage makes it possible to process the fluid which contains an analyte eluted from the sample matrix. The fluid and the analyte contained by the fluid are passed from the first region to the second region within the analysis device, and in the second region the analyte is detected by SERS.
The document further describes SERS-based detection architectures for the second region, including implementation with SERS-active particles/nanoparticles, optionally in dried or immobilized form, and SERS-active optical elements. In particular, a second analysis region is configured as a hollow-core optical fiber with SERS-active coating for receiving an analyte-containing sample. The kit architecture includes the sampling device, analysis device regions, a radiation source, a detector, and optics to determine the analyte presence or/and amount based on SERS measurements.
Claims Coverage
The partial content provides two independent claims: a kit claim and a method claim. Across these independent claims, the core inventive structure combines a sampling device with a sample matrix and an analysis device with a first region, a second region, and a downstream processing stage that enables processing of eluted analyte fluid for SERS detection. Additional main inventive features are captured by dependent refinements such as SERS-active particles, microfluidic structures, monochromatic radiation source with optics and inelastically scattered radiation detection, and hollow-core optical fiber implementation.
Downstream processed eluate routed from sample matrix to SERS detection region
A kit comprising a sampling device with a sample matrix and an analysis device having a first region for introducing the sampling device and a second region configured for detecting the presence and/or amount of the analyte via surface enhanced Raman spectroscopy (SERS), wherein regions are interconnected by at least one processing stage located downstream from the sample matrix that processes the fluid containing an analyte eluted from the sample matrix while the fluid and analyte are passed from the first region to the second region within the analysis device.
Receiving, introducing into multi-region analysis device, and determining analyte by SERS
A method for detecting an analyte in a sample comprising receiving a sample via a sampling device comprising a sample matrix and an eluent for eluting the analyte from the sample matrix, introducing the sampling device into an analysis device comprising at least a first and a second region with the first region configured for introducing the sampling device and the second region configured for detecting the analyte in said solution, transferring the analyte in a fluid from the first region via at least one processing stage located downstream from the sample matrix while processing the fluid and/or analyte and passing them to the second region, and determining the presence or/and amount of the analyte in the second region via surface enhanced Raman spectroscopy (SERS).
Both independent claims require a sampling device with a sample matrix and an analysis device with a downstream processing stage that processes eluted analyte-containing fluid so that the analyte presence and/or amount is determined in a second region via SERS.
Stated Advantages
Not explicitly described in patent.
Documented Applications
Not explicitly described in patent.
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