Antigens and T cell epitopes from cockroach and methods of making and using same
Inventors
Peters, Bjoern • Sette, Alessandro
Assignees
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Publication Number
US-11505581-B2
Publication Date
2022-11-22
Expiration Date
Abstract
The specificity of CD4+ TH responses of German cockroach (Bla g) antigens, and whether differences exist in magnitude or functionality as a function of disease severity, is disclosed. Also disclosed are novel German cockroach allergens and epitopes.
Core Innovation
The invention relates to a pharmaceutical composition that comprises a peptide and an adjuvant. The peptide comprises an amino acid sequence selected from specified cockroach T-cell epitopes, including TIPYYTKKFDEVVKA (SEQ ID NO:1), HDDRLGFLTFCPTNL (SEQ ID NO:7), KNRTTIRGRTKFEGN (SEQ ID NO:8), DRKMYWQFKMDKIQI (SEQ ID NO:9), IRGRTKFEGNKFTID (SEQ ID NO:11), and additional peptides defined by SEQ ID NOs 12, 14-18, 20-23.
The invention relates to the discovery and characterization of CD4+ T-cell responses to German cockroach (Blattella germanica) allergens, including determination of T helper subset balance across Th1, Th2, Th17, Treg/TR1, and Tfh. It links the magnitude and functionality of anti-cockroach/allergen responses with clinical asthma severity, and includes allergen/epitope reactivity and immunodominance differences across subject groups.
The composition and cockroach epitopes are used to induce immunological tolerance and suppress allergic responses, including immunotherapy/specific immunotherapy (SIT). The disclosure also describes diagnostic and detection approaches that support immune modulation assessment, including contacting subject cells with peptide sets and determining immune modulation using cell-contact assays, including T-cell assays and ELISPOT.
Claims Coverage
The claim set contains four main inventive features anchored around composition, immune modulation, tolerance induction, and subject-cell-based peptide identification for cockroach allergy treatment.
Peptide composition with selected cockroach T-cell epitopes and an adjuvant
A pharmaceutical composition comprising a peptide consisting of an amino acid sequence selected from TIPYYTKKFDEVVKA (SEQ ID NO:1), HDDRLGFLTFCPTNL (SEQ ID NO:7), KNRTTIRGRTKFEGN (SEQ ID NO:8), DRKMYWQFKMDKIQI (SEQ ID NO:9), IRGRTKFEGNKFTID (SEQ ID NO:11), NDIEKRVPFSHDDRL (SEQ ID NO:12), VLEKLEAGFAKLAAS (SEQ ID NO:14), NYAIVEGCPAAANGH (SEQ ID NO:15), GIRIYVDVVLNQMSG (SEQ ID NO:16), RWRQIFNMVGFRNAV (SEQ ID NO:17), NIACLLHNKYDSTKS (SEQ ID NO:18), LNIFTNNLGRINTHV (SEQ ID NO:20), KTPVLEIDGKQTHQS (SEQ ID NO:21), PAYFKMNSPSLWKYN (SEQ ID NO:22), and PKSMLLNIFTNNLGR (SEQ ID NO:23), and an adjuvant.
Immune response modulation to a Cockroach allergen by administering the composition
A method of modulating an immune response to a Cockroach allergen in a subject by administering an amount of the pharmaceutical composition sufficient to achieve the modulation.
Inducing immunological tolerance to a Cockroach allergen
A method including inducing immunological tolerance in a subject to the Cockroach allergen.
Treating cockroach allergy with peptide identified by subject-cell immune modulation
A method of treating cockroach allergy in a subject by administering an effective amount of a peptide identified by contacting a subject cell with the peptide and determining whether the peptide modulates immune activity of the contacted cell.
The claims cover a composition that combines specific defined cockroach-derived peptide epitopes with an adjuvant, with dependent claims refining use toward immune modulation against cockroach allergens, including inducing immunological tolerance and treating cockroach allergy in a subject.
Stated Advantages
Induces immunological tolerance and suppresses allergic responses.
Modulates immune responses against cockroach allergens.
Induces immunological tolerance related to cockroach allergen immune responses.
Treats cockroach allergy in a subject.
Documented Applications
Treating cockroach allergy in a subject, including a human subject.
Modulating immune responses to a Cockroach allergen in a subject.
Diagnostic or immune assessment use via contacting subject cells with peptide sets and determining immune modulation, with application to allergic responses including asthma and allergic rhinitis.
Immunotherapy/specific immunotherapy (SIT) to induce immunological tolerance to cockroach allergens.
Therapeutic treatment of cockroach allergy in a subject, including a human subject.
Use in the context of modulating CD4+ T-cell responses to German cockroach allergens relevant to clinical asthma severity.
Diagnostic sets mapping epitope sequences to allergens/proteins, including sets associated with Asthma vs CNT.
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