Protective barrier compositions and uses thereof
Inventors
Monsul, Nicholas T. • Berkes, Eva A. • BOEHM, Frederick T.
Assignees
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Publication Number
US-11457633-B2
Publication Date
2022-10-04
Expiration Date
Abstract
The subject invention provides compositions and methods of enhancing the barrier function of surfaces and/or objects using biochemical-producing microbes and/or byproducts synthesized by the microbes. Preferred embodiments of the invention provide compositions, and the methods of using the same, comprising a Lactobacillus sp., and/or bioactive extracts thereof, derived from human microbiota and capable of growing in biofilm phenotype.
Core Innovation
The invention is directed to barrier-enhancing compositions and methods that use Lactobacillus-derived biofilm products to make a surface less susceptible to microbial contamination. Inactivated Lactobacillus spp., grown as biofilm, are applied to a surface such that the treated surface becomes less susceptible to microbial contamination. A particular embodiment specifies inactivated Lactobacillus fermentum Qi6 (Accession No. PTA-122195) as a biofilm-derived source for the barrier function.
The disclosure emphasizes a barrier function that operates on living and inanimate surfaces with antigen/pathogen-agnostic activity, including inhibition of pathogenic biofilm growth/adhesion and promoting commensal growth. A specific barrier protein is identified as Qi611S (SEQ ID NO: 1, encoded by Qi611s, SEQ ID NO: 2), including formulations based on cultures and biofilm-derived fractions such as lysates and purified/lyophilized fractions.
The document further describes barrier activity associated with Qi601S and Qi611S fractions, and reports stability and sustained barrier activity claims. The stated formulations include inactivated Lactobacillus biofilm compositions and isolated protein compositions comprising SEQ ID NO: 1, with activity described across different surface types.
Claims Coverage
The partial record includes three independent claims. Across these, the inventive features cover inactivated biofilm-grown Lactobacillus compositions applied to make surfaces less susceptible to microbial contamination, and a separate barrier method using an isolated protein comprising SEQ ID NO: 1.
Inactivated biofilm-grown Lactobacillus composition for enhancing a barrier against microbial contamination
A method for enhancing a barrier against microbial contamination of a surface by applying to the surface a composition comprising inactivated Lactobacillus spp. grown as biofilm, such that the surface becomes less susceptible to microbial contamination.
Inactivated L. fermentum Qi6 biofilm composition for enhancing barrier susceptibility
A method for enhancing a barrier against microbial contamination of a surface by applying to the surface a composition comprising inactivated Lactobacillus grown as biofilm, wherein the inactivated Lactobacillus is L. fermentum Qi6 having Accession No. PTA-122195.
Isolated protein comprising SEQ ID NO: 1 for providing a barrier against microbial contamination
A method for providing a barrier against microbial contamination of a surface by applying to the surface a composition comprising an isolated protein comprising SEQ ID NO: 1.
The claim set in the partial content centers on applying inactivated biofilm-grown Lactobacillus compositions, specifically L. fermentum Qi6, to make surfaces less susceptible to microbial contamination, and separately providing a barrier using an isolated protein comprising SEQ ID NO: 1 (Qi611S).
Stated Advantages
The treated surface becomes less susceptible to microbial contamination.
Barrier performance and/or longevity are enhanced on an object's surface.
Stated durability and sustained barrier activity over days.
Stated heat stability and pH tolerance for barrier compositions/fractions.
Stated inhibition of pathogenic biofilm adhesion and reduction of microbial effects in described models.
Stated protection in additional described contexts, including a coronavirus context and AChE protection context.
Documented Applications
Enhancing a barrier against microbial contamination on anatomical surfaces of a subject, including skin, intestine, stomach, lung, eye, mouth, nose, ear, trachea, vagina, and esophagus.
Enhancing barrier function on protective suits, medical implants, personal hygiene products, bandages, clothing, eyewear, footwear, and masks.
Enhancing barrier function on surfaces of objects selected from vehicles, boats, ships, cars, trucks, tanks, airplanes, and trains.
Providing a barrier against microbial contamination on medical/consumer-contact surfaces, including medical instruments, countertops, tables, appliances, chairs, dishes, utensils, protective suits, bandages, clothing, eyewear, footwear, headwear, and masks.
Use cases supported by described figure contexts include inhibition of MRSA biofilm adhesion and MRSA reduction on ex vivo human skin, and anti-adhesive activity on intestinal cell models.
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