Fluorescent standard strip
Inventors
KIM, Sungrak • LEE, Huije • Sohn, Hosung • YOO, Seungbum • SOHN, Mi-Jin
Assignees
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Publication Number
US-11442011-B2
Publication Date
2022-09-13
Expiration Date
Abstract
A fluorescent standard strip, according to the present invention, is for testing the reliability of the fluorescence intensity of a fluorescent strip used for an analyzer, the fluorescent standard strip having an effect whereby, for devices using a fluorescent substance, the inter-device deviation in fluorescence intensity is reduced, fluorescent signal linearity per fluorescent concentration level is secured, and device reliability may be tested by periodically checking faults, errors, deviations, etc. of the devices. In addition, the fluorescent standard strip, according to the present invention, has an effect whereby, even in an environment and state where light is frequently irradiated, the reduction of fluorescence intensity according to time may be minimized, i.e., the same level of fluorescence intensity may be maintained for a long period.
Core Innovation
The invention relates to a fluorescent standard strip for testing a fluorescent strip used for an analysis device for reliability as to intensity of fluorescence. The fluorescent standard strip includes a substrate, a fluorescent layer stacked on the substrate and including a fluorescent substance, and a masking layer stacked on the fluorescent layer to partially block fluorescence exposed to the outside from the fluorescent layer. The intensity of fluorescence for the fluorescent substance of the fluorescent standard strip is higher than that for a fluorescent substance of the fluorescent strip to be tested.
The masking layer includes patterned light transmitting portions and light blocking portions, and the light blocking portion is formed as a boundary between light transmitting portions. Patterned microstructures and line features are used as a masking pattern, enabling control over fluorescence emission toward outside for maintaining fluorescence signal linearity versus concentration while reducing inter-device fluorescence intensity deviation.
In testing, the fluorescent standard strip is applied to the analysis device, and a fluorescence detection value is measured. A determination is made as to whether the fluorescence detection value falls within a required standard value range, thereby checking reliability of intensity of fluorescence, and the measured value is optionally compared to a required standard value to calculate and correct an error based on a deviation value of the analysis device.
Claims Coverage
The independent claim defines a fluorescent standard strip for testing fluorescent strip reliability of an analysis device based on intensity of fluorescence, with three inventive features in the core layered structure and masking arrangement. Additional claim coverage specifies emission wavelength matching, patterned masking-layer structure, and reliability determination with optional error correction based on deviation.
Fluorescent standard strip having higher fluorescence intensity than the tested fluorescent strip
A fluorescent standard strip for testing a fluorescent strip used for an analysis device for reliability as to intensity of fluorescence, comprising a substrate, a fluorescent layer stacked on the substrate and including a fluorescent substance, and a masking layer stacked on the fluorescent layer to partially block fluorescence exposed to the outside from the fluorescent layer, wherein an intensity of fluorescence for the fluorescent substance of the fluorescent standard strip is higher than that for a fluorescent substance of the fluorescent strip to be tested.
Emission wavelength range matching the analysis device fluorescent substance
The fluorescent substance of the fluorescent standard strip has an emission wavelength within an emission-wavelength range of the fluorescent substance used in the analysis device.
Patterned masking layer with light transmitting portions and light blocking boundary
The masking layer includes patterned light-transmitting portions and a light-blocking portion that blocks fluorescence, with the light-blocking portion as a boundary between transmitting portions.
Light transmitting portion formed by spaced microstructures reducing fluorescence transmission
The light-transmitting portion has a pattern formed by spaced microstructures that reduce the fluorescence transmission of incident fluorescence from the fluorescent layer.
Reliability test by measuring fluorescence detection value and checking required standard value range
Testing reliability by applying the fluorescent standard strip to the analysis device, measuring a fluorescence detection value, and determining whether it falls within a required standard value range.
Error correction by comparing detection value with required standard value using deviation
Further compare the fluorescence detection value with the required standard value to calculate and correct an error for a deviation value of the analysis device.
Overall claim coverage centers on a fluorescent standard strip with a substrate, fluorescent layer, and masking layer that partially blocks outside-exposed fluorescence while providing higher fluorescence intensity than the fluorescent strip to be tested. Additional dependent coverage specifies emission wavelength range matching and a patterned masking-layer architecture, and includes method steps for within-range reliability determination and optional deviation-based error correction.
Stated Advantages
Minimized inter-device fluorescence intensity deviation by using the masking layer and masking pattern to tune transmittance.
Maintains fluorescence signal linearity versus concentration.
Minimized time-dependent fluorescence quenching and reduced fluorescence decrease over repeated measurements.
Periodic fault/error/deviation checking by determining whether a measured fluorescence detection value falls within a required standard value range.
Error correction by calculating and correcting an error based on a deviation value.
Documented Applications
Testing a fluorescent strip used for an analysis device for reliability as to intensity of fluorescence by applying the fluorescent standard strip to the analysis device, measuring a fluorescence detection value, and determining whether it falls within a required standard value range.
Correcting errors in an analysis device by comparing the fluorescence detection value with a required standard value to calculate and correct an error based on a deviation value of the analysis device.
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