Microfluidic system and method with focused energy apparatus
Inventors
Appleyard, David • BETTHAUSER, Jeff • FAUST, Marjorie • Larsen, John • SHAO, Guocheng • Xia, Zheng • Zhou, Yu
Assignees
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Publication Number
US-11415503-B2
Publication Date
2022-08-16
Expiration Date
Abstract
A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
Core Innovation
The invention provides methods for producing a sexed composition of sperm comprising non-human animal sperm by identifying a subpopulation of sperm from a population of sperm in a sample fluid. The methods include staining the population of sperm in the sample fluid with a dye and defining a gating strategy for differentiating the subpopulation based on one or more physical characteristics of sperm, identifiable within the population based in part on the dye staining.
The methods include measuring the one or more physical characteristics for the sperm in the population of sperm and measuring a span distribution of the sample fluid based on the defined gating strategy. Sperm in the subpopulation are identified based on the measured one or more physical characteristics and the defined gating strategy, and the sexed composition of sperm is generated comprising the subpopulation identified based on the measured one or more physical characteristics and the span distribution.
A related aspect defines the gating strategy used in producing the sexed composition of sperm by determining one or more desired physical or genetic characteristics, setting gating parameters to differentiate the subpopulation based on the dye-stain-identifiable characteristics, and marking the desired physical or genetic characteristics in a plot or a graphic representation. The method further includes measuring the one or more physical or genetic characteristics, measuring a span distribution based on the defined gating strategy, identifying sperm in the subpopulation based on the measured characteristics and gating strategy, and generating the sexed composition.
Claims Coverage
The document portion includes four independent claims. Across these, the inventive coverage centers on defining and using dye-stain-identifiable physical or genetic characteristics together with span distribution and gating strategy, including plot or graphic marking, to identify a sperm subpopulation and generate a sexed composition.
Sexed sperm composition from dye-stain-identifiable gated subpopulation using span distribution
A method for producing a sexed composition of sperm comprising non-human animal sperm by staining a population of sperm with a dye; defining a gating strategy differentiating a subpopulation based on one or more physical characteristics identifiable based in part on the dye staining; measuring the one or more physical characteristics; measuring a span distribution based on the defined gating strategy; identifying sperm in the subpopulation based on the measured physical characteristics and the defined gating strategy; and generating the sexed composition comprising the identified subpopulation based on the measured physical characteristics, the span distribution, and the defined gating strategy.
Defining a gating strategy with desired physical or genetic characteristics, dye-staining, marking, and span distribution
A method for defining a gating strategy for use in producing a sexed composition of sperm comprising non-human animal sperm by determining one or more desired physical or genetic characteristics; setting gating parameters resulting in a defined gating strategy, wherein the physical or genetic characteristics are identifiable within the population based in part on dye staining with a dye; marking the desired physical or genetic characteristics of the subpopulation; measuring the one or more physical or genetic characteristics; measuring a span distribution based on the defined gating strategy; identifying sperm in the subpopulation based on the measured characteristics and the defined gating strategy; and generating the sexed composition comprising the identified subpopulation based on the measured characteristics, the span distribution, and the defined gating strategy.
Sexed sperm composition with gating strategy locating desired characteristics in a plot and span distribution
A method for producing a sexed composition of sperm comprising non-human animal sperm by staining a population of sperm with a dye; defining a gating strategy differentiating a subpopulation based on one or more physical characteristics identifiable within the population based in part on the dye staining; identifying one or more desired physical characteristics of the subpopulation in a plot of the one or more physical characteristics; measuring the one or more physical characteristics; measuring a span distribution based on the defined gating strategy; identifying sperm in the subpopulation based on the measured one or more physical characteristics and the defined gating strategy; and generating the sexed composition comprising the subpopulation identified based on the measured one or more physical characteristics, the span distribution, and the defined gating strategy.
Defining a gating strategy with desired physical or genetic characteristics marked in a graphic representation and span distribution
A method for defining a gating strategy for use in producing a sexed composition of sperm comprising non-human animal sperm by determining one or more desired physical or genetic characteristics; setting gating parameters resulting in a defined gating strategy, wherein the physical or genetic characteristics are identifiable within the population based in part on dye staining with a dye; marking the desired physical or genetic characteristics of the subpopulation in a graphic representation of the one or more physical or genetic characteristics; measuring the one or more physical or genetic characteristics; measuring a span distribution based on the defined gating strategy; identifying sperm in the subpopulation based on the measured one or more physical or genetic characteristics and the defined gating strategy; and generating the sexed composition comprising the identified subpopulation based on the measured one or more physical or genetic characteristics, the span distribution, and the defined gating strategy.
Across the independent claims, the covered subject matter combines dye staining with gating strategies that differentiate and mark a desired sperm subpopulation based on identifiable physical or genetic characteristics, measures those characteristics along with a span distribution, and generates a sexed sperm composition based on the identified subpopulation.
Stated Advantages
Documented Applications
Producing a sexed composition of sperm comprising non-human animal sperm.
Use of the methods in producing a sexed composition of sperm by identifying a desired subpopulation based on dye-stain-identifiable characteristics and a defined gating strategy including span distribution.
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