Escherichia coli bacteriophage Esc-COP-7, and use thereof for suppressing proliferation of pathogenic Escherichia coli
Inventors
Yoon, Seong Jun • JUN, Soo Youn • Paik, Hyoun Rok • Son, Jee Soo • Kang, Sang Hyeon
Assignees
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Publication Number
US-11412760-B2
Publication Date
2022-08-16
Expiration Date
Abstract
The present invention relates to a Myoviridae bacteriophage ESC-COP-7 (accession number KCTC 13130BP) isolated from nature, and a method for preventing and treating infections from pathogenic Escherichia coli by means of a composition containing the Myoviridae bacteriophage ESC-COP-7 as an active ingredient, the Myoviridae bacteriophage ESC-COP-7 being characterized by having the capability to specifically kill Escherichia coli, and genome expressed by the SEQ ID 1.
Core Innovation
The invention relates to a Myoviridae bacteriophage designated Esc-COP-7, isolated from nature and having the ability to specifically kill Escherichia coli. The genome of Esc-COP-7 is represented by the nucleotide sequence of SEQ ID NO: 1, and Esc-COP-7 is deposited as accession number KCTC 13130BP.
The invention addresses preventing and treating pathogenic Escherichia coli infections. The background motivates the use of bacteriophages instead of antibiotics in view of antibiotic resistance, and positions Esc-COP-7 as an antibacterial agent with distinct specificity.
Compositions comprising Esc-COP-7 as an active ingredient are described in formats including a disinfectant, a drinking-water additive, and a feed additive for use against pathogenic Escherichia coli. The document reports that Esc-COP-7 kills 9/10 pathogenic E. coli strains, does not kill selected non-target bacteria, and inhibits pathogenic E. coli growth in culture. In an animal model, the document reports reduced diarrhea index and lowered piglet mortality after oral administration, including delivery via feed and drinking water, and also reports environmentally friendly applicability.
Claims Coverage
The independent claim covers an administration-based treatment of a pathogenic Escherichia coli infection in an animal other than a human using a defined Esc-COP-7 composition. The claim set includes 3 inventive features tied to the specified phage composition characteristics and the specific administration/format for the composition.
Treating pathogenic Escherichia coli infection in an animal using Esc-COP-7 composition
Administering to an animal other than a human a composition comprising a Myoviridae bacteriophage Esc-COP-7 having an ability to specifically kill Escherichia coli and defined by the genome represented by the nucleotide sequence of SEQ ID NO: 1.
Defined phage identity by deposited accession number
Using the Esc-COP-7 composition where the genome described by SEQ ID NO: 1 corresponds to Esc-COP-7 deposited as accession number KCTC 13130BP.
Administration format as feed additive, drinking-water additive, or disinfectant
Administering the composition in the form of a feed additive, drinking-water additive, or disinfectant.
Overall, the claims are directed to administering to an animal other than a human a composition containing a specifically Escherichia coli-killing Myoviridae bacteriophage Esc-COP-7, with identity defined by SEQ ID NO: 1 and the deposited accession number KCTC 13130BP, and optionally specifying administration as a feed additive, drinking-water additive, or disinfectant.
Stated Advantages
Specifically kills Escherichia coli.
Kills 9/10 pathogenic E. coli strains.
Does not kill selected non-target bacteria.
Inhibits pathogenic E. coli growth in culture.
Reduces diarrhea index in a pig model after oral administration.
Lowers piglet mortality when delivered via feed, drinking water, or disinfectant.
Distinct antibacterial specificity.
Environmentally friendly applicability.
Documented Applications
Preventing and treating pathogenic Escherichia coli infections.
Use of a composition comprising Esc-COP-7 as a disinfectant.
Use of a composition comprising Esc-COP-7 as a drinking-water additive.
Use of a composition comprising Esc-COP-7 as a feed additive.
Animal use in a pig model, including oral administration and delivery via feed or drinking water, for reducing diarrhea index and mortality related to pathogenic Escherichia coli.
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